DAF-8

WormBase ID: WBGene00000904

WormBase description: Exhibits RNA polymerase II transcription factor binding activity. Is involved in several processes, including dauer entry; negative regulation of dauer larval development; and regulation of transcription by RNA polymerase II. Localizes to cytosol and nucleus. Is expressed in excretory cell; hermaphrodite distal tip cell; intestine; neurons; and ventral nerve cord.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TTGAGCCATTGCCTCAAGTC

sgRNA2: GAAGAAACTATTATCACTGA

5'HR: GCATGCTCAATAAAAAGTAGTGACGTACATCCATCTGAAACAAAAATAACAATATGACGCCTTCTTAGTAGTACTTATGTTAGTTTCTATTAATGGACTTTTTTTTAAAATAAAAACTCAAAATAGTCAAAGAAATTCTAAAAATGGACATTTGCTAAAATTTAAGAGAAACGATTTCAAAAGATGAAATTATACTTTCCTCCCAACTTTTTTGCTTTTGATAAATTGCTGGCCGAGTTATAACTATTTCTAGGTCACGTATCAGAAGCCGGGTGAGTCTTGAGATCTTCGTGATGCCTTGTCATATCTAAATGAACGAAACTTGAATAAATTAATTAGGAGTCATCGGCTTCATTGAGTTCAGAAAAATTCTGTATAAAACAAAATCAAGATTCAGGTAAATATCTAGATTTTCGATCATTTTGGAACCGCGTTCCTGATATCGAAAAAACTTCGAATCCATTTAAAAGTTGAATAAATCAAATGAAAACTTCCTCTCATTTTTCTTCATTTCTCTCTCTATTTTTCCTTGATTTTATAGGTGATGACGATTCTGATCAACACAAAAATCTTATCACAAACTGTTGTTTCTTATCATTTTTTACACGTTCATACCAGTTTTCATTCCGAGTTCTTGAATTTCATAATTCTTTCATTTCGCCTTTCTATGTAGATATTGAAACTCCGCCCATTTGAGTAGCTCACCTGACTCTTATGACTTACCAGAAGGTTCTATTATCATTTTGGATATAATTCTGTTTTCTGATTCAAATTTTTTTTGAATTTCGGCAATGCACAACGAAAAATATTAATTTCATATATAAAATCATCTGTACTATTTATTATTCTTTATCCCAAATCAGTCCTTTTTTGTGGTCGTTGCTTTTTAAGCTCGGCCCATTTTTTTGGATTTTATTCTAGCCTAACATCTCCTCCCATATTTTTTTTAATTCTTCGAAATTTCAGTTTTAAAACTACGAAAGGTACC

3'HR: CTGCAGATGGACGATTTTCCTTCACCAGAACCTACTGATCCTAGACTCGAGGCAATGGCTCAAAAAGTGTTAGAAGAAACCATCATTACCGAAGGAGATGTATGTTTTTGAAGATTTGAAACTTTTTTTTTGAATTTTCAACACAAATAGTCTCGCTGGCTACTTTTAAAAATTAAAAAGCCATCTGCAAATTTTAAATTTCATATTCTAGGCCAGCCGGCGAGCAAGTAAGTGAACAAGAAACTTTAAAAAAGAGCAGAGTTGAGAGATACCGTATCTTGATCAAACATCAAATGTCCAGACATATTAGCGAATTTTTATTTTAAAATATTTAGAATCAACCTACATAAACATTAACTTATACGTTTTCTAATTTTAGAATGTGTCTCTCAAATTAGCCTTTTCTTTTAAAACAGGAAACAATTTTTTTTCAAAAATAGTAATACTTAAAGAAACTGAAAGGATATTTACGACAAAAGGTTTTAATGTATGTTGGAGACTCGAAAAGATCCAAAAAAAAAATAAAGAGAAAATTACAGGAAAAGAGAAAAGCCCATATCTTGTTTTTTAAATTTTGTTATAGTAAACTTTTCCTAAGAAATTCAAATCCTTAAAATTGCCCTATTTGAAGTGTCAAGCTATCCTCAACTATTTTTTAGGGAACAATCTACTGGGCTCTCAAAGTTACCCGATTACTCTCTAGAGTAGCTAAAAAACACCAGTGCTTTGAAGCTTTCTACGATGCCGTTATTAAAGGTGATCCAAAAACACGATGCTGTCCTGCACACAATGAGAAACTCATTGGAAACTTTGGACGAGCTATCATGTGTGTCTTGAGAGCATTCAGATTTCCGGTTATTCGATATGAATCTCAAGTGAAATCAATATTAACGTGTCGACATGCATTCAATTCCCATAGTCGGAATGTTTGTCTGAATCCGTATCATTATCGATGGGTTGAACTACCGACGTGCCAAGTTCCACCAATTATTGTAAATAAGGAATTGGATTACGGTGAACCTCCGATTCGACTAGT

KI verification primers: DAF-8-NKIF: 5'-AATAGTTTATTTATTCTGGTTCCTCATG-3' DAF-8-NKIR: 5'-TCATCGTCACATTTGGCACAT -3' DAF-8-NKIF2: 5'-GAACACCCCAAAATTAGGTTCC-3' DAF-8-NKIR2: 5'-AGTCAACGTTTTTGGTAAGTTTGC-3'

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Strain name: SYS605

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; daf-8 (devKi177 [mNeonGreen::daf-8]) I

Expression pattern: Neuronal-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: DSC-1, FKH-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: DSC-1, FKH-8