LIN-48

WormBase ID: WBGene00003033

WormBase description: Is an ortholog of human OVOL1 (ovo like transcriptional repressor 1). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific; DNA-binding transcription repressor activity, RNA polymerase II-specific; and sequence-specific DNA binding activity. Is involved in hindgut morphogenesis and nematode male tail tip morphogenesis. Localizes to nucleus. Is expressed in ciliated neurons; interfacial epithelial cell; intestine; labial sensillum; and tail. Human ortholog(s) of this gene are implicated in posterior polymorphous corneal dystrophy 1.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GTAATAACACTAATGTCTCG

sgRNA2: CAGCTCTGCTCAATTTCAGA

5'HR: GCATGCTGCCTAATATACTTGTGTCATATATCCTTACAATTATATTAATATATCTACATGTAAGAAGAAGTTTCATGTCCCTTCTTGCATCATTTTTATATTTTCTGGCAATTTGCAGAAATTATTTTGGGGGATTTTTAAAAATTTTCAACAAAAATAAAAATAGTTTTTCAAGTGAACCAAATATTTATCTATGTTTCTAGCTCAAATTTTGAAACAATTCGATTAGGTAATTTTTCTCTCAGATATTTCAGTTTTCCAGCCAAAAACTGAAGATTTTTCTGTCTTACTGAAATTTCCTGTTATAAATGCAACTTTTTCAAAACTATATGCACAAAAAACACAAAAAAATATTTAAAAAAATACAAAATATTTTTTTCGGATTTTTTTTATTGTTGAACTAAAAATGGTTTTGGGAGAGTTTCGTGAAAATTACCCAACTTTTTTTTGTGGTTTAGGTCTAGGCTTAGACTTAAGCTTAGGCTTAAGATTAGGCTCAAGCTAGGGCTTAGGCTCAGGCTTTGCTTTAGGCTCAGTCTTCCAGCGTAGGCTTCAGCTTAGGCATAAGCTTTGGCTTAAGCCTAGGCTTCGGCTTGTGGGAACGCTCAATAGTTTTGTGTACCTCTCCCCTAAACCATCCCAGAAAAATCCTAAATTTCACTTTCGGTTTCATGATTATCCTCTCCCTACCGTGATTTTGTGCTCTCGGAGCCAACACCTCCATATTCCAATTATTCCCGACATCCGAGACATTGTACCTATTATTATCTGGCGCCGAGTATTTGAAATAATTGCCGGATTGGATTTCTTCGGTGATAACACACCCCGCCCCGACTCGGACGCTTCCGCTTGTTGTTCCTTGTTTGGAAAAAATTCCACAAAATCATCACAAAAATTTTCAGCTCTGCTCAATTTCAGTACC GGT

3'HR: CTGCAGATGGATTCACGGGTATGGCTGCCGCTGATTGGAGCGCATTTGCTGCCTCGCGATATCAGCGTTATTACTCAGGTATTTGAGTCTTGATTTCAAAAAAAAAACTTGAAAGTGTGAATTCAGATGATTGCAAATAATAATAATACATCAATTTCGAGAAGTAGCACCAATAGCAGCAAACCCGAAGAGAAGAATTCGAAAAAGGTAGGAATGGAAAGTTGGTGGCCTAGAAAATTAGAAATCTCGGCCATCTTGAAATTTTTATCTAATATTTTTTGTTCTGAAACCGCCTGAAATTATCTTTTTCGTTTTGAACCGCGTAGCCTAGATAGACACATTGGCAGGCACAATACTTATCTACGATGAAAGGAACATTTTCAGTTTCTCATTGAACGATTTCTCGATGATGATCCTTCACCACCAGCATCGATTCTTTCGCCATCACCCAAGGCAGCCATACCCAGTGTAAGACGCTAATCAAGGAAAATTCTAAATTTTTTTTTTCTGATTTTCAGCCAATTATAAATCCAGCAGTGGAGTTTGTAAACGGAGGCTACGGAGTCAAAAACCCCCTGGCTCCGTTGATCAGCTCCTTCGAAACCACCTCAATTCCGTGTTCAACAGTATCTCCTTCATCCCTCATCTCCTCCAGCAAAGACGAGTTCCAAGATTCATTAACCTGTCACATTTGCGGCAAAAAGTTCGGTTTACAACGTCTTCTGAACCGTCACATAAAGTGTCATAGTGACCTGAAACGGTATCTATGCACATTTTGTGGAAAGGGTTTCAACGATACATTCGACTTAAAAAGGCATACCAGAACACATACAGGTACCTCTCAACACTTTAAAGTACTCCTCTACCTCTGCCACCAATTCATAACCACTAATTTGTCGTTCTTCTTACCCTCCGTTGACTTACGGTTGGAAGACAATTGTCTGCGTCTGTTTGTCTAGCACACTGACTAGT

KI verification primers: LIN-48-NKIF: 5'-CTCTCTTTCCAATTGATGCTTCTTC-3' LIN-48-NKIR: 5'-GAATATTACGCTTCGCTACAATGG-3'

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Strain name: SYS638

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lin-48 (devKi195 [mNeonGreen::lin-48]) III

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-34, CEH-36, ELT-2, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, ELT-2, ZIP-8