KI information : 	mNeonGreen knockin at N-terminus
sgRNA1 : 	AACACTTACCTTTTCGATCG
sgRNA2 : 	GATGATGACACTTCTGAAGT
KI-homologous recombination fragment-5'arm : 	GCATGCTGAAATGGAGGATTTGGAAATTTTCTGAAAAACGGAAAAAGGTTGAAAATTATGAAACATAATTTTTATTTTATAAACTGACCCACAAAACTCTTCTCTTTTCCATTTTTTATCACTTGGCAATACTTCAATTCGAGAAATGAACTCGAGCATTTCCTCCATCAAAAGTCTGTAAATTTTTATCTAGCAGTTGTAATATCCAATTTCTTATATCACTCACTTTCCGTACTGATACGCAATCTCCGTAACATAAATTTTCCCAGAAAATCCGGAACCCTCTGTGTAGAACGGCAAACCGACGAATGATTCATAGTTGGACACCAAAATTGCGTCGATTGTATCCATTTTTAGCATATCCGTCTGAAATGCGTCTAGTATTAAATTTAATTAATCTCCATGTTTACAAACCGATACGTGGAAAATTTCAGGTGGCGATTCGACGTAGACACGGTTTCCGAGCTCTTTCAGATACGGTATCTGAAACTTCTTGGCGATCGGAGCATTTTTGATTCGCGGGCTGAAATTTTTTGCTTTGAAAAGAAACGACGTCACTTTTCCTTGATTTACCTTTGATATACATGTGGGAGGAAGCTGAAAAATGGTGTGAAATCAATCGGAGTGTCCATAAGAATTCTGGCATTTGGCCATTCTAGCAGAAAACATGGTTTTTGTGCGTACACCGAGAAGTTTGTCTGAAATTAGTTATTTATTCCATCTGTCAAGTAGTTTTTTAATCAAAAACCTACGATATTCATTATTTTAGTCTTGTTTCAGCGCCCTTCCATTAATTTTTAGATAAATACAAAAAATAAGTTCGAATTAAATTGGATTCTGCGCTAAGTGCGGGTGCTGACGCATTACGCGATACTGTTTGCGTTATTTTCGAGATTTATTTTCGAAAGTGTCCCATTCAAGTTTCAGTCAGTTTCAGCCAAGTTTTCATTGATTTTTCTCATTTTAATCTTGAAAGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGATGTCTTCCAAATACTCAAAAAAACGAAAACAGTTCGATGATGATACCTCCGAGGTTGGAAAAAATCGAGATTCATCAAGATCTCCATCTCCTCCACGCTCAAAGAGGTAAGTCTTTTTGTTACTTTTTCTTTTTATTAATTCTGTAATTCACAGGTGAAATTGCAGATCTGCGAAACAGCGAGAGCCTGAGGATAATGGAGAACCGTAAGTATAACACGGAGAGCATTTCATTCAATAAAATAATTATTTTTCAGGGCGGATTCGATGTCAATCGAAGAAACAAACAAACTGCGCGCCTCGCTTGGTTTGGCTCCTCTTGAAACTAACGACAATAAGGAACGTGATGCTAATGATGGAACCAATGAGAAAATCTATAATGAGGATGGATTTGAGTTCAGACATCGAAAAGCTGAGAATATTTCTGATAAGAAAAAAGAAAAAGATATCAAAGAGAAGCTAGAAATCGCCAAAGCGAAACGAGATGTTTATTCAAAAGTCCTGAAAGCGAAGAAACTCGCAGATTCTGATGAAGAAGACAGTGCAGCCAGTTTTGTGAACAAAATGCGTCAGAAAGAAGATGAAGCCAAAAAGGCCGCCGATAGAGCCGCAGCTTTTGATCAACTTGACGAAGAGCTTGGTGTCAGTGCAATCGTCGATAAAGTGAAAAAGCCGAAGAAACCAAAAACCAAAGGGCCATCTGATACAGCTGGAATGGTTATTGGTCATGGGAGAGAGGCTTTTATTGAAGGTGATCAAATTTTGGTGCTTCAAGATAAGGGAGTTCTTGATGATGGAGACGAAGTTCTCGTGAATCCAAATCTGCTAGACAATGAACGTCACGTCAGAAATGTTGAATTGAGGAAACGGAAGGATCCTAATCGGAATTTTGATGAAGATGTCGACGAGTTTGGAAATGTAGGGATTTTATTTTAAAAGTTTAACTGATTACTAGT 
KI verification primers : 	F19F10.9-NKIF: 5'-GTGCATATCAGTTGTCGTGTAGTAGG-3' F19F10.9-NKIR: 5'-CCAACGTCTCATCGTACTTTGC-3'