KI information : mNeonGreen knockin at N-terminus sgRNA1 : TCATGAGATTCTAGGCATTC sgRNA2 : AATTACTGATTTGTGCACTT KI-homologous recombination fragment-5'arm : GCATGCACAAATGGAATCTATGAAAACTGGGCAGCAACGAAATTTTAAAATTACAGTATTCTTTAAAGGCGCACGCCCTTTAGCAATTCACAAAAAAAGAGTGTCGAAACCTGTTACTGTATTTTTTGCGCAAAACTGCTAGATCTTGCGTATGTGTAAAATGAAAGAACGTATTTTTTCTGAAACTTCGAAAAATTTGGAAAAAAAATGATTTTTTTTACAGACTCATCCTTTTGAAAATAAATTTCATTTCGATTTTTTTTTGAGTTTCAGTTTCAGTTAATTTCTCTGAAATTTACCTCTAATATAAATCTGCTTAAATTAGTCCGTTCAGCATCTCGTTTCCTTGTTAGAACACCTTGATCAATCGGAGTCTCGATGATTTTCAGCACGGCATCTCGATCACATTTCTTGATGAGAAGATAGGTTTCACCTGGAAAAATGGATGGAAATTAGTAATTTCGTTCTGAAAACTTGTAGCAGTTAACTTTTCTGCTGATTTTGCTAATTTGATTTATAAATGGATGTTCTGATAAATTATTAAAAATTCTGGTATTTTCCTGCTCCTTTGAGCCGTGAATTTCTTCAAAAGCGGGTTTTTTCTTCAATCGAATGCTAAATAATTTCTCAAAACACTTCAAATACTCCTTCCTGTTCTTTTTCTCATAATTCTCAGCAATTAGTAACCAGAAGCTCCTCCTCCTTCGTGGTACGGTAGACGTCGATCTGCGAAACCAACCAAAAATGGGCGTGGTTTTCTCAGCGATTCGTCAGGAAATTGGATCATTTGCCTCCTCCCGTTTCTCTAGTGTGCTCTCGACTACTGCACACCGGCCAATTCATTTTTATATCAGCCTCCTCCTTCTCGTCTTCTCATTCCAATTTTCCACTTTGGGTAATCTTCTTCAATTTTTCCAGATAAGCAGCCACTTTTCTCTTGTGGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATGGCGGTGGACGAAATGCAGCTCGCTGCATCAATGCAAATGAGCAATCAAATGAGCAATTCACAACGAATGAGCTTCGCAATTCATGAGATTCTGGGCATTCAAGTAATATCATTCGTATTCGGAAGTGCACATATCAGTAATTTTTAGGGAAATGCATATCTATCTCACGGATATTGTCCTCAGGGAACATTTCTCTCAAATCAACCCAATTTCATGGACATTGGATCCTGTGGAGTATCAGGGTTCGACAGACAGCTGTGTGGTACTGACTCTATTGGAAATGGTAGGATATTCATAGTTCATAGATTCAAACGAATTTTCAATTTTCAGCACCTGCTCCACTGATGTATCGAATGCCAATCTCAACATCAGAGGTTATAACTTCGGAGCCCCAGACTCATGTCAGCTCATCAAGCATCTTATCATCAGCAACTACATCAAATTCTTCTGGTGGTGGAAGCAGTGGTGGTGGTGGAAAGGCAAGCAAGAGAAAGAAGAGAAGACATCGCACAATTTTCACTCAATACCAGATCGACGAACTCGAGAAGGCTTTTCAAGATTCTCATTACCCAGACATCTATGCCAGAGAAGTCCTAGCTGGAAAGACAGAATTGCAAGAAGATCGGATTCAGGTCAGTTGATTTTTCATTGTAGCCCCTTACCAATTACGAAAACGGAGCCCCTTTTATCGGTCGGCATCCATCTTTTTATTGGTGCTAATTTCCTCCATTTTGCTCTTCTTGACTCTCGGAAAGTTAGAGGACATGCTCCCCCACAAGAATAGGGGCAGCAATGTGTCAATTGAATTTCTTGAATTTCCTTCAAGACAAGTCAAGACACGGCCAATTGGCTGATAGTATTGAGTGAAACCCAAAATTCTAAAGGGTTCTCACGCTTCTTCGCTTCTATTGGCTTACTGAAACCTGAGACCTAATTATCTATTAGATGACAAATTGTACTAGT KI verification primers : CEH-10-NKIF: 5'-TCTTCCGAGTTGTGTGGGACTTA-3' CEH-10-NKIR: 5'-CCATATAGCCCATATTCTGCCATTA-3' CEH-10-NKIF2: 5'-TCTTGTTGTCGTGAGCCCAGT-3' CEH-10-NKIR2: 5'-CACGACGGTTTTGGAACCATAC-3'