KI information : mNeonGreen knockin at N-terminus sgRNA1 : AGCTTCATCCTCTTCGACGT sgRNA2 : TTTCGCGGCGGGACCCGTTG KI-homologous recombination fragment-5'arm : GCATGCCAGAATATTAAAAAATTCAAGCCGCGATGCGACCGCGCCGCACTGGGATCAAAAGGCGCGAGTTTAAATTTCAAAGAGCGATTTTTAAAGTTTTTCAAAAAATACTAAATACATAATTTTCTTTTTCAAAATTTTATTAATCTCATCGCATTTTTCTTCCGTACAGCTGCCGTCTTTTTCTCTGCGCTCTCTCGCGTCTTCTATGCTCACTCTCACATTCTGCGTCTCCGCCGCGTCTCGTACTCTCTCGCTAGTCCTACTCACACGTTTTTCACTTGTTTTTTTGCCTAAATTAACGGATTTTGCCACTTTGTCATCGATTTTCAGCGAAAATCTATAGCCGCATCGTGCTTGGCGTATTTCTTTGACTCAGTAGCTTGAAAATCCCGAAAAGTTTCGGAAATTTTGATCCTGGATCCAGGATCCGCGAAAAAGTGGATAAGATTTTGTTTTTTAAGGGTAGCAATTCTAAAATCTAGAGAAAATAGATGGAAAAATTGATTTCCAAGCTATTTCTCACTTAAAAATCAAGAAATTTGCGAAAAGTTTCCAATTTTGCAGAAAAAATTTTGGAATTTGAGCTACATTTTTGATTTCTGGATATTTTTCGCAATTAAAGTATTAAGATTGTCTAATTGGAGAGGTGTGAACGTAGAAATTTGAATTTTGCGAAATTTTACTGCTAATTAGCTTAAGTTTACAAAAAACACCTTATGTACGCCGGGCGCGTTGCGGTCGCGTCGCGGTCTATTTGAAATTTTATGATTTTTCGAGTTTTTAGAAAAAATTAATGCTTTAATTGAAGATTTTAAGCAAAATTAGTCGTGGGAATTATCACAATTTTCTCCTCGCGTTGCGGTCGCGTCGCGGTTTTCTCAAATTTTTTGTTTCTGAAAACCACTATTTCCCTAAAATATTATAGATCTCCAACAAAAGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATGCGCCCAAGAAGGTCCGACTCTACAACAGGTCCCGCCGCGAAATCACTTCCCACCTCTGACGTCGAGGAGGATGAAGCTGCGCCGGATCTCAAGGCAGAATCCGAAGACGTGACGTCATCAGAGGAAATGCAGAACAAGAATCCCGTCAGTGCTCCAGAAAAACGGGCAAAAACCGCCTCATTTAGCCCACCGCCCGCACTTTCGCCCGCCCACTCAATCCAAATGGAAAACGAGGAAATCGAGGTGAAAATGGAGGTGGATAGTGATGAGGAGGAGAAAAAATTACCAAAAATCACTGAAAAACGAGCCAAAATGGTGTATAAGGTGAGACAAGCCTGCACGAGAAACGCGCCAACGTCCAGCTGGTGCATTTCTCGATACCGTACTTCAAAATCCTCGTTTTTTGCAGAAAAAACCCGAAAAAGAGAAAGAAGCAGTGCCATTTATCGATCCAAATGCTCCAAAAAGAAATCGATCTGGATATGTGCATTTTATCATGTAAATTATCGATTTTTCGCGAGAAAATCCCGAAAATCCCCAATTTTTTCAGACATCGCCGAGCTTCATACTCAAAAGCCGTGATGTCACAGAGAGAAATTAATATATCGCTTGCGGCGGACTGGCAGAAGCTTACCGCCGAAGAACGTATCCCGTTTCAGCAGAGAGCCGACGAGGAGAAGGTCAAATATTTGGAGCTGATGGAAGAATACAAGAAAACTGACTCGCATAAGGAATTTCAGAGTTCGTTGTGCTAAAAATCGAGTTCTCCGCAGTTTTTTACCTGTAGGAAGTATAGGAAGCGTTAATTTTGACAACTTTCGGGTTACGGTAGCCCCAAAAGTACGTAAACACCGTACACTCTACGCAAAAATGCACAGAAATTAAAAATTTTGCCAAAACCGTCTACATATGGGTAAAAAGGGTCGCTGAGCTCGATTTTCAACTAGT KI verification primers : HMG-20-NKIF: 5'-CTACTGTAGCTTTCACTAATTCTCCCC-3' HMG-20-NKIR: 5'-AGCCATTTGACTGAGATGACAGG-3'