KI information : mNeonGreen knockin at C-terminus sgRNA1 : ATTGATATTAGCGAATGCAG sgRNA2 : ATAATTGCACAGATATCTTT KI-homologous recombination fragment-5'arm : GCATGCTCAGCTTCAACAGCAGCAAAATAAGATGAGATTGATGCAGCAACAGCAACAGGAAATGCAACGAATTGAACAGCAAAGGAGGCAGCAGATTATGCAGCAACAACAACAGCAGCAGCAACAAGAACATCAACGACAACAGATGCTTCTACAGCAGCAGCAACAACAACAACAAATGCAGCAACATCATCAAATGAATGGAGGTGGACAATTTGCGACGCAGGCACATCAGCAGGCTGCTTATATGCAGCAAATGCAGAGAATGGAGCAGATTCGGTGAGTTTTTTTTCCAAATCGAGAAAAATTCATTAAATTTTCATTGAATTTCTGAAATTTTTCAAAGTTTTTAAATAATCTAATTCGAGAAATCTATCGAAATTTGAAAAAAAATGTATTTAAAATTTATTGATAACAAAGCAAATTACATTTTTTTATGATAAAATTCGGGAGATTTAATTTTTTTTCAAAAATAAAAAATTACAAAAAAAAACCAGTGTAATTCTGACTTAAAAATTTAGATATTGATCATAATCTAGCATAAAAAATTTTTTCAATAGAGCATAGATTAAAATTTTAAAACCCCATAAAAAGTAATTTTAAAAAAATTATCGGATTTTTCTAAAAACGTTTTTTCGATACTCATATTTTAATCAAAAATCAAAAAAAAAAAGGTGAAATTCAACCAATTTTTGGAAAATTCCGAAAAGAAAATTAACTGAAATTTGAACATTTTTGGTAAAAAAAAACCTATAAATGTTTCTGAAATAAGCTCAATTTTCCAGACACCAACAACAACAAGCCCAACAACATCAACAGGCTCAACAGCAACATCAGCAGCAAGCTCAACATCATCAAATGGGTTACGGTATTCCGAATGGATATCCACAGCAAATGCATATGCATCCACCGGCCTACGGAGCCCATCACATGCCACAACCTACTGCCTTCGCTAATATCAATGGTACC KI-homologous recombination fragment-3'arm : CTGCAGTTTTAGAAAATTGCGAAACGAAAAGATATCTGTGCAATTATTCATTTTTTTGTGAATGATTTTTTCCCATTTTTTCCATTTTTCCCCCCGATCAAATTCCTTGCCTTTAAGAGTGGAGTAGCGGTGATTTTGCTTCTTCTAAAAATCTGAAATCGTCTTAAATTTAGAAATTCCACAAAAGAAAAATGAAAAAATACGCGATTTTTCAATTTTGGCTGAATTCAAACTGCAACCAATCAGCGATTCGCTCCGCCCACTTTTGAACCAATAAGATAAATTGGTCTCATTTTGGAGGGAAGTTTAAAAAAATTGAGAAATCACGATTTTTTCGCTTTCTTTGGTGGTTTTTTGTGCAAAATTTATTTTTTTCTCATTCCCACTACTCCACCTCCAATGAGGTTATTCATGTGGTGTAGAAAAATGCAAAAAGTTGTTTTTATACATTTACCCCCCCCCCCCCCCCCCCCCACCTACTCGTGAGTGCCGAAAAATGTTTAAAAACAACGCATCCATTTTTGCATTTTTCGGCTTTTTTTTTGCATTTTTTGACATTTTCTGCATTTTTGGCATTTTTTGGCATTTAGCGCAGAGAGCTGTGTTCACGCAGGATGTCGGAAAAAGCAGAAAATTGTCGAAAATGCCAAAAAAAGTCGAAAAATGTCGAAAAAATGCAAAAAATGAAAGCGTTTCAAAAACATTTTTAGACACTCATGAATAGGGGGAAAATGTATAAAAATACATTTTTGTATTTTTCTACATCATATAAATAGCCCCATAAGCACTGGAATAAGCTTCTTCTAGTCTTCTATCCCCCGCTCTAGTTGTGTATTGTGAAGCGTCTTTTGTCTCATGGCAGGCCATGTCCCATTCCTTGATCATTCCCCTCGATTCTCTCCATCATTTTATGTACCGACTAGT KI verification primers : SEL-8-CKIF: 5'-AAGCAGTCTCCGATGGAAATGA-3' SEL-8-CKIR: 5'-GTGAGGGACGAAGAGTAGATCAGAA-3'