KI information : mNeonGreen knockin at N-terminus sgRNA1 : ATCCCAGCATCCATCTACGA sgRNA2 : ATCGGTCATAGTTCCAAGAG KI-homologous recombination fragment-5'arm : GCATGCAGGAGGCGGAAATCCTGGAGAAGCCCAACGAACCTATGGAATCAGAAACGCTAGAGAGGCACCAACAGCTGGAAAAGAACGAGGAGCCTAGAGAGTATGAAGAGGACAAGAAGCTCAAAGAACCTGGAGAGCACGAAGAAACGGAAAAGCTTGAAAAGCCCAATAAGCTTGAAAATTCCAAAAAGTTCATGGAGCTTGAAACGCAGGACGACAATGAGAAGTTCGAGGAGACTAAAGAAATCAAGACGCCCATGGAGCCAGTGAAGCTTGAGGAGACGGAAAAACTGGAGAAGCCCAGTGAGCTCAAGGAGTTCAACGAGCTTGCGCAACTAAATGAGCTCAAAGTGCTGTCACAACCCAAACGGTCTGAGAAGCCCGAAAAGCCCAATGAGTATGAAAGGTCCAAGAATCTAGATGATGGTTCCATTAAGCTTGAGGAGCATGAGGATTTGGAGGAAGACAAGGAGCTCAAGGAATTTGTGAACCCAGGAAAGTTCAATAGACCCAGTGAGCTTGAGCTTGAAAAACTCGGTAAGCCCGAAGAGCCTAAGGTTGATTTGGAGGAAAATGAGGAACAATTGGAACTCGAAGAGCCTAAGAAGAAATAATTAACAAACTTTTTCCGTATTTTTGTATTTCTTACTATTTATCTCACATCACTATCCCTAATAGTAAAAATGTTTGTCTGTATGTAGTTTAAAATGCTCCACTCTCATTCAGAAGACTAAAGAAAGAGCACGTCAAAATAGGAGGGCACAGAGATGCAAACAGCCTGTGGGTGTAGGTTATAGAGCACACGAGATGAAGGCGACGCAGGCGACAAAGTCCTCTGGATATGGACAGTCGTTGTTGCAGGTCCGCCAGTTTTAGTTAACAGTTTTAGTTGGTTAGTTTCGATAGTTTTGCCTCTCTTGGAACTGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATGACCGATGCTCAATCTCGTGATGAGAAGGGGATCCCAGCCTCTATTTATGACGGGATCTTCAAGAACAATCGCCAGATCAAGCCTGGAAATCCAAATGAATTGAAGATGGTAATGGGCTTGAAGTTGAAGGAAGTCAATAAAGACTGCGCTGACGCTGTTCTGCGCGTTTCCAAAGCGACACTCAGTTGGGAAATCGTCAGGGAGATTATGAATGCGACAGAGCGTTTGGTAAGTTGTCAAACTAGAAAATCAAGTCAAAAAATCATTTTTCAGATTCAAGAAAGGGTCAGTGATCCGACCGGTCAAGTGGCTATTGCCGTCGATCCCCCGAGTAGTTGGTCCAAAAGTGTCACGGATTTCCTGGCAAGCTGCTACAAGTTGAGCCCGTCGCCGAACTTCAAATTCCTTGAGCTGCTGGCTATTCTCACTGGGAAGTCACAGTATCTGGTAGGAGGATATCTATAAGATCTGGGGTTTTATACTCTGATTTCAGATTCTTCAGTGGTTTGACACAGCTGAAGAGGAAAAGCGTGAGAAGAATCAGAAGCTTGAGACGGCTACGTTCAAGCGTCATTGTCCATGGCGGAAGAGACATAGACTGGCTAGTGTTGAGGGTTTTGCAGGCGACTTACATATCCAACTTTTTCAGATCCAGCCCCTCTCGAACGCCACCGCCATCCCGTTCCTGGAGGCTGCTTTCCAAAAATCGTCTATCCCCGGAAGCGTTGAGCTCTACGACCTTCAGCTGAAAACCAACTGGTTTATTTGTGAGGTAACCGCGAATTTTCGATCGACCTTTTTTTTACTTTTCTATATTTCAGCTAAAGGCCTGGTTCACTGCAAGACGGGTTGCCGAGGAGATCAAGGGGAAGTTGAGTGAGCTGACGGGAGTTCAGGGTGTAGCAAAGGATCAGAGTTGAAGGATCCGGGAAGATATTTTTTCGTTAAAGTTTTATTACTAGT KI verification primers : CEH-82-NKIF: 5'-GACGAGCTAGAAGAGATCAAAGACC-3' CEH-82-NKIR: 5'-AGCCTAAGTCTAAACCTATGCTTAAAAC-3'