KI information : 	mNeonGreen knockin at C-terminus
sgRNA1 : 	TTTGTAGGAGATGTGACTGG
sgRNA2 : 	TCAGGATCTACAAATGGACT
KI-homologous recombination fragment-5'arm : 	GGATCCTACCCTTTACAGGCGGTTTGAATTATGAAGCTATACTGAACAATCCCGTCTTAAATGCTACTATTGCTGGACATTTGCCAAATCCTCTTGCATCGAACATTTCCTTGCTTCAAAAGAGTATTAGCGCAAAACTTGCAGCCGCTGGAAACATGCAAACAGTCGAAAAAGTGGAGACACCTTTAAATCTTTCAAAAGACACCCCATCTCCAACTGCTATTCCACAATCTCCACTATCTGGATTTCGGCTACCATACTCACTTGGGACAAATTATGGAAGCGATGGTATTATTCTAAAGCTTTCACTATAAAATTAAAAGTTTCATATTTTCAGGTCAATTATTTAACAATTGCTCACCAAATTCCAGTGGTAAGAGCACGCCTGGTAACACATCAGTAACTAGTGAAGTAGCAACTCCACGACCACAGGCCAAATCACCAAACCATATCAAAAGACCAATGAACGCATTCATGGTATGGGCTCGTGACGAGAGACGAAAAATTTTGAAAGCGTATCCGGATATGCACAACAGCAACATCTCGAAGATCCTCGGTAAGTTGTCTTGAGAGCAGAAAATAGATTCGATCGTCGTTTCATCTGACAAATTACTTAAATTACTTTTAGGATCTCGTTGGAAAGGTATGAGCAATTCCGAGAAACAGCCATACTATGAAGAGCAATCACGGCTGAGCAAATTGCACATGGAGCAGCACCCGGACTATCGGTACAGACCACGTCCGAAGAGAACATGTGTGATTGATGGAAAGAAGGTTCGAGTCAACGAATATAAGACAATCATGAAGACTAAAAAAGATTTGATGTGGGGAGATGAGCCAGGATTTTCTCAACCATCAGTAAGTGAACTATTCAAACAATTTTTCAAACCGTAATTTTATCAGGATCTACAGATGGACTTAGCATCACATGTAAATCTTCTCAACGACCTCACACAGCACCATCACCAATCACATCTCCTACAAACAGCTGAAGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGCTTTAACCAAGCGTGTCATTCCATCTGATCCTCCAAACATTCTCTGTATTTTTTAAAACCCCCACCTTCGAATTTTGTCGTGTTGTTCTCATGCCAGCTTTACTTTGGTACTCCTCACCCACCACATTTACTTACCTGGTCCATCATGTATTATTACAGTATCTCAATTTTTATTTTTTTTAGCAGATCGGTTTCTTGATTTTCAAAATCTTGGACAATCAAAATCTGCTTGCCGGTGGACCAATGCATTTTTTTTCCATTCATTTTCTCTTTCCTTTCCTCATACAATAGCATAATCTGAAGCGTTATTTCCTTTTCTATGTGTTTCCTTATTGTACCTCTTTCAAAAGCTTCCCACACTCCAAGAATCTCAGCGTTTCCGCATTGTTTCCTACATATTCTGAAGACTGCATTTCAGTTTGATTTACTGTGTTCTTCATATCAATTCCTTTCCTATTCATAATAGGCCTTTTTTCCATTTTTTTTCCTCCTTTTACTGCATTGTGTTCCCATTTTCCACCTGGCACCAGATCTTTTCCTTTATCTTGTTGCAACCTTAGGCTAGTCATCAATCAAACATCCACTCCCGTTTCCATCAAATTTTTACTCTATGCATGCATCTGATTATGGTTCACACTCAATTTTCTGATAAATGTCATATTCAAGAACAAGAGAACAAAACATCATAATATTCGGATTATGGTAGGAAATGTGCATTATGAGTTACAGCCGGAAATGAGAAGATCATCGTAGAATGATTTTGAGAATAGGGGTACAATTGCTATGCAGTTGGAAAAATCAAATGAACCCTTCACACGAAAGTAAGATACATCTTAAATATTTCTTAAAACTTTTCCTTCTAGTTTTCTAATATCTTCTAATAGTACTGCGCTCCATTACATTATTACTAGT 
KI verification primers : 	EGL-13-CKIF: 5'-CACTCCTTTTTAATGGTACGTTCCT-3' EGL-13-CKIR: 5'-GTTTTGTTCCATTCAAAAATTGTCA-3'