KI information : mNeonGreen knockin at N-terminus sgRNA1 : GAGATGATGCAGTGGCCTTC sgRNA2 : "GATGAGTTATCACTCATTTT BAZ-2-NKIsg3: TTTCGATGGAGATGATGCAG" KI-homologous recombination fragment-5'arm : AAGCTTTTATTGAACTAAGTCTAGAAACTTAATTTTTTTAATCTCGTGTTTTTTAAAAATAATTTTATTCGATCTACAATTTTACCAAAATCACCGCTTCCCTCATATGGCATCTGAACCCTCCCTTCCTCCTACATAAATTTCTCAATACGTACCAAAATATTTCATCACCAAGTAACCGCATACACCATTCGCATTTGTCCTCCTATTCCTCCACTTGTCCTCTTCTTTACTTTTCATCCACTTTTTGCGTCTTCCAAATATCGTTCTTTACCAACTGTGAGTGAGAGAGAAGGGTACCTCCTGCCGCCTGCTGCTGTAGCCATTTCCCTCTCTCGCTGTGGTTCAGTATACTCGCTGATTGACACATCTGTCCTTTTTCATTCCCTCCGTGTTGTCCTGGTCATCGTTCAATCGTTTTCCTTCATATTCTTCCTCACCATATCTTTCTCTGCTTTGCCTTCGTTCAATTTCTGATGGTACGATTTTGTTAAAGTGTTTCACGGGACAAACTTTTGAAGTCGCGACGTTTTCTAGAACCGCATGAACTCAAATTGTCACATGCATCTAAATAGACAACAATTTTTGCTCACCTTTCTTTAAAATTTCGATATGAATTTCCATAATTTTCAAAGTTCTATTCTACTAAACTTAATAAAGACTAAGCATGCAAACCTGAACCTAAAAACTTGAACGTAACCTAAGCTATGAGCGAAAACTGTTGTCAATTTACGAATGCATATTCTCGCGACATAATGATATTACTCCTTAATAATACATAGAACACTCATGCTCACAGACCTCTAGATTCAAAGGCCTTTATGGTGTCTGACTATTTTTGTCTTCATCACACATTTTTCGAAATTCTCACTTTTTATTCAACTTCTTGACCCTTTTAACTTTCATTTAATATTGCTAAATTTCAGAATTGTACATGTAAATTCTTTCCAAAAACC GGT KI-homologous recombination fragment-3'arm : CTGCAGATGAGTGATAACTCATCTAATCAGTTTCTACTTCTGCTAGCAGCTGCACAGCAACAACAGCAACAACAACTTCTACAGCAACAACTTGCAAAGATTCAGAAGGCTACTGCCTCATCTCCATCGAAATCAACTAATGGAACATCAGCATCAACGTCGGCTGTCCCGTCAACTTCTGGGACATCGTCATCTCAAAATGAAGCAGCTCAGTTGCAAAATTTAGCAAAAATGCAGCAAATTCAGCAGCTGGCCCAGTTTGGAGCACTCATGGCTGCGCAGAAGAAACAACAAGAAAAAGCGGCAGCTGATAAGGCAAAGGAAAAAGAAAAGGAGAAGCAAAAGGCTGCCGCAGCAGCTGCCGCCGCCGCAGCAAAAGCCTCAGCGTCTACTTCCTCCGCATCAGCAATTCCTGGATTGTCTCCAGAAATGCTCGCAGCATGGCAACAAGCAATCCAGATGCAAGCTCTCCAACAAATGATGATGACTCCACAGAAATCTCAAATGGAAGAAGCTATCAAGAAAATGATGGATATGGCAAAGAAGAAGCCAGCTGGAGTCGCCTCAACAAGTTCAGCATCTACAAGCTCATCCACACCATCCACATCATCAGCTTCAATTACATCATCTAATAACAATGCAGCGAACAACGCTGCGAGCAATATGATGAACAATGTGATGTGGCAATTGGTGGCAGCACAAATGCAACAAAAACAGCAACAACAACAGAAGGATACTCAGAAAAAGGCGGTAAGATATTGCTTTTTATATTATTTTTGAGAAAAATGTTGAATACTTTCAGGATCAAGCGAAAAAAGCGAAAGAGCTTGCCAAACAACAACAGAAAGAGCAAGATGTGAAGAATAAGCAACAAGAGGAGATTTTGAAGTTTCTTATGGCTCAACATCAGCTGAATCATCAGAAAAAGCATGAGAAGAAACTAGT KI verification primers : BAZ-2-NKIF: 5'-GAGGTAGTGGTGACCATGAAAGAGTA-3' BAZ-2-NKIR: 5'-TTCTTCAGGACTGTCCGATTCG-3'