KI information : 	GFP knockin at N-terminus
sgRNA1 : 	Y75sgRNA1: GGAAATGAACATGATGCATA
sgRNA2 : 	"Y75sgRNA1: GGAAATGAACATGATGCATA
Y75sgRNA2: TCAACGTATACTATTTTTGC"
KI-homologous recombination fragment-5'arm : 	"GCATGCGCGCTTTGCATCGATCCATTGTATACAAATAGAAATAAATATAGGAAGGAAGGAAAAAGATTTGAACTGATCAGGGGAAGTATCAAATGTTTTACAGACCAGACTCTGTGGAATAAATTGAGACTTGATGAAAATGAACGAAAAAATCTGTTTAGGATATAGACAGGGGTGTGCGGCAAATTCGGCAAATTCAGCAAATTCGCCGCACACCCCTGGTACAGTATACCATGTTAAATGTAATCAATTACTTACCACCTTACCGTAGTATAACTGATGATATTTCTGAAGCCCTAACAATTCAAAATAAACGGCTCACTGAAAAAGCAAACCTTGACACTTCCCAGTCTCTCTATCGTTTTACTAAACTAACTGAAGGGGATAAGAATGCAAAAAATAGGCAACAAAAAGAGGTGTCTCTCCCACCTCTTACTATGTGTTGCTGCTGCTGCTCCACCCTTTTTGCTCAAGTCAATGTGTTGTATGAAATATGATTACATTTCAATCTTATTACCTTTTTTGCCCCTTTTTCTCTCCACACAACTCATGTGTACCTATTCACACCCCCCCCCTCTCAAACACGTTTTTCATCTCTTCTTCCAACAACACTTAGGGTTTCTGGAAAAACATTTTCTTCTAGTGACTTTTTTTTTTGAAACTTCATCTCAATATCAGTAGATCACAAAATTCTGAAAATGCGTATGGTGCAACACATTTGACGCGCAAAATATCTCGTAGCGAAAACTACTGTAGCGCTTCTGTCGATTTACGAAGCTCGATTATTGAAAATTTTTGAAATTTCTCAATTTTAGGCTTAAGCTTTTGCTTAGGCTTAGGCTTAGGCTCAGTCTTAGGCTTAGGCTTAGGCTTAGGCTTATACTTAGGCTTAGGCTTATACTTAGGCTTAGGCTTATGCTTAGGCTTAGACTTAATCCTAAGCTAAGGCTTAGGCTTAGGCTTAGACTTAATCCTAGGCTACTGTAGTTTTCGCTGCGAGATATTTTGCGCGTCAAATATGTTGCCAATTACGCATTTTCAGAATTTTGTGTTCCCGTGATATGCTCTATTATTCCCTTTTCGTAATTTTTCAAACAATTTTCAATCAACGTATACTATTTTTGCAGCGGGAAATGAACATGACCGGT
"
KI-homologous recombination fragment-3'arm : 	CTGCAGATGAATATGATGCACATGGGTGCTCCAATGAACGGGGGCCCGCCCCCACCTCAAATGCTCCAAATGCACCATCCCCTGCCTCACCACGTGGCAGTTTTCGCCCACGGTTCATCGTCACAAAACAGTCAGGCGCCGCCCCTTCAGGCCGCTTCGGCTACACCTCCAGTACCGTCGAGCATGAATCGATCACAAAGgtaagtgtgtggcgggtgtgtaggggggtcacttttttttgggagatggttatcaactttctggcaatcggcaatttcggcaatttcggcacttatcggttttagaaatttccggcaatttcgaaaatatcggcacttgccggttttttgaattttttgtcagtttcgacaatttcatcacttgccggttttggaaattttcggcaacttcggtaatttcggcaattgtcggttttggaaattttcggtaatttcgacaatttcggcacttttgccggttttggaagttatcttcaatcgggaacttcggcacttgttggttttttaagttttcgacaattgccggttttggaaattttcggcaatttcggcacttggcgattttggaaattttcagcaatttttgcaatttcggcaattgcaggttatggaaattttcggcgattggcaatttcggcaattgccagttttggaaattttcggcgatttcggcaattccggcacttgccggttttggaaatttccggcaattggcaatttcggccattgccggttttggcaatttcaggcagttggcaattccggcacttgccggttttgggaatttccggaaagcgacaattccggcacttggcgattttgaaaattttcggaaatttcggtatttccggcaattcccgaagttgccgaaaattcgattgccgcacatacctggtttcaagttcccatatttttcctattagactagcattccgattgctcaagatctgatatctcagtACTAGT
KI verification primers : 	Y75-KIF: 5'-AGTTTTCGCTGCGAGATATTTTGCG-3' Y75-KIR: 5-TTGACGGCTTGTCTTGAATTTTAGCA-3