KI information : mNeonGreen knockin at N-terminus sgRNA1 : GTGGGGTCTAGAAAGAATGC sgRNA2 : TAAGTTTATCTTCGCGCCAC KI-homologous recombination fragment-5'arm : GCATGCCACAAGTACCAAAAGATGTTACCAGTTAATCTGAACTGTACGTGTGAAGCAAGTTTAAGATCACAGCCAGGAAAATCTAAATGCAAAATGAACTATCAAAAGTAGTCACTGTTACTGAACTTTTCAGATAGTGTTTTCCATTGTGCTGATTTCAAATCAGTGATGTATCAAATATTAAAACACACACAATGTACCTATTAGACCTCATCATCTTTTAGTCAGAAAGCTGAAACATCATTCGGGCTGAAACTCATTTTTAGCTTTAGTCGCTAGTTTTTGCACGCCGAAGCAGCATGCTATTTTTGATTTTTTCTGCGGTTTTACACTAGCATAGCTAGTTATATTGAATTCTATTCAGTGCTTTTTTATCTGAAAATCATTGCTCGTTTGGATCATGTTGCATTGCGTTGTTACTCTGCTTTTAGGAAAAGCCTAGTGTGCATAGTTTGCATTCGGCAGTTTCGGGATTTGTCTGAATTGTTTTCTTCTCACCTGAATGCCTCATTTCCGAAACTTTTGCTTTTCGTCTTTTCCTCGAAGCTGCTCGTTTTCAGCTTCATCGTCCCCCATTATCACACTTGATTGGCTTCAAAAAGGTGGATTGCACGGACCACACTTCTCAATACAAAACCGCGTGGTGTCATCCATTCTATTCCCTTCTTCGACACGCCCCGCCTTCCACTTCGCATGGGCGTGACTAGACCAATCAACGAGTTTGCGACGTTTAGTGACTGCAGGCAACAGGACCCTTCCAACCCACTCATTCACACTAAACTACTGTCTAAAAGTCATTCAATTTGACTATTACAAAACTTATTGTATTGTTACATCATCTGATAAGGTTATCATAACAGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATGTCAATGTTGGATCTCCGTCAGTTTCTGATTCCTGCTTTTTTCCTTGATCCAACCAACCAACTTGCTGCTCAGAGTTCTTCTGGTGGCGCGAAGATAAACTTAAACAGCTCTTTTCGCATTTCTGATCTGCTTGATCCAACTTCACACTCAACCAACGAACCAGGTAATCCATTTTGCCACCATTTTCCTAAAGTTATTGCATTTATTGTATGTTCGTTCAACCATCTCTAACACTTTAATCTACATAGACTTCCATGAGAATGTTGAATGTACAACTAACCTGAAAAATGATATTTAACGGATTAACGCTCCAGTAGTCACATGGACGGTAATCTGTAATAGTAATAGGTCAACCAATTATGATCCTTTGTTCTCCTCTTACCTATACAAACAGTTTTCTTTATTTATTTGTTATTGTAGATCCATCTCCTACCTCCATTAATTCCTCGGAATCGCCATCATCCCCTAGAATTGGGAGCCCGAATACCGAAAGATCTGTGAACGAGAGTCCGACATGCGTGGGATCAAAAAAGGCTCGGAAGGCGAGAACTATCTTCACCGACAAACAACTTCAAGAGCTAGAAAACACCTTTGAGAAGCAGAAGTACTTGTCTGTTCAAGATAGGATGGAGCTGGCTCATCGAATGGGGCTAACAGATACTCAAGTAAAGACATGGTATCAAAATAGAAGGTTGGTCGGCTATATTCCTTAATTAGGCAAACTTTTTTTCTTTTCTCGAAAAACAACAACGTGTGGCTCTTTGCGCCAGCAGTTGGGCGAGAATTGAGTCTAAACACGCCACTTTGTGTTAATTGTAGTGCATTAGCGAGAAGCTTCACCCCCATCGAAATTTATCTTAATCAAAGACTAGT KI verification primers : CEH-31-NKIF: 5'-AGTTTAAGATCACAGCCAGGAA-3' CEH-31-NKIR: 5'-TTGAAGAAGTTGGGACGA-3'