F19F10.1

WormBase ID: WBGene00017598

WormBase description: Is an ortholog of several human genes including ERG (ETS transcription factor ERG); ETV5 (ETS variant transcription factor 5); and FEV (FEV transcription factor, ETS family member). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Human ortholog(s) of this gene are implicated in acute myeloid leukemia and craniosynostosis.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: CTTCCGTACCGCCAAAGAGT

sgRNA2: CATCCCCTTTCATCTTTGTT

5'HR: GCATGCTCATCGTACTAAAAAATATGCTTCAAAAAGTCAATGAGCCGATACATGGATTCACTTGAATTGTTTTGGATTAAACTTTTGCTTCTTTCAAATGTTGAGCCTTGAAGTGAAAATATATTTTCAAACAGCATAATTTGGCAAAAATGGGAAACTGGGAATTGTTCAATTTGGGGGAAGTCCTGAAAATTATTGAAAATTGCAATTTATTGTCTTGGTGAAATTTTAAAACTTTAACCATACTTTAAAACGGGACTATGACAAACAATAATTTCAAATACAATTCATTTTAACAGTTGAACCATAATAAAACCAATGTTTAATTTTTTCTTGACCCTAAAATATCTTCCAGTGAAATCATGTAAAATTTCCGGTTGTGACACTTGAAAAACTTTTCTAAAAGTTGTCATGAAGTTTTTTTGTGAAAACATAAAATAAAATAAATAATGAATAGTTCCTAAATAGGTGAAAATATCACAAGATGTGATGTTTCAAAAAAGTATTATATTTCAAAGACTCGAATGGAATAACTTAGAAGCCCATAATAAAAATTAGTCATTATTTTGTTCGAACGTTTCCGCCGAAATTCTATCTGATTTTATGAAGTCCAGAACAATTTCCGAGAGCTCACATTGTTGTAAAAAATGACCCGAAAAAAATTCAGATCGTCTTATTTACGGTTGTATCAAATTTTTGTTTTAATATTATACGTTTTATCTAAAAATTCTGAAGTGAGCTTACACATCCTTTTGAATGTTTCTGTCAAAATTGGATTCGTAAAGTAAGCATTAAAGATTCTTTAATCAAACATTCAAAGTTCTCCTAGTTATATTCAGTTCAGTATGTTCATATGTTTCGTCTTATATCCCTGTACTAGTATGTGACCCCAATCAATATTCCAGACCTTCTCCCTGCAGCTCCACATTGCCTCCTCTACGCCCAACAAAGGGTACC

3'HR: CTGCAGATGAAAGGGGATGTTTCCCCCATATTCTCACCAAAACATCTCCAGTTCAAACGACCGACCCTCTGGCGATACGGTAGAATACAAAAGGTCCGCCTTTTTTTCTTTTTGCATAGCAGTCAGGTATGAATACCACACCATTTTCAGTCATTCCAGCTAGTTTGTGGTTACCGAGTCAGTGACTTCCTTTTCTTTTCGGTTTACATTTCCGGTACTTAGAAATTTTAATTCTTCGATTTATTCTCTTTTCAGTGAAACTATTTGTTTTCCAATTTTCCTGATCTCCCTTCATTATTTGCTGATTCAAAGTTGTTTTCTGCACCTATTTATTATTGCCAGCAGTCGTCTGTTCACCATGGCTGTAAAACCATTTCCGTTTGAACGCATTGTCACATAACCCCGGCGGTCTATTAGAACTGATTGTAAAACTATAAGAAACTGTGAGAGAGTGATTGTTTTCGTGGTTTTTACTCATATTTATGACTTCGTGTTTCCTTGTTTTTACTTATGTATTTTCTAATCCCAACTAATTTCAGATTTCAATAATGTCATCAAAACGTTCGTCCCCAACTGGAAAACTCCGTCTTCTGAGCTTCCTTCGTGACCTTCTTGAAGACGAATCGAATTCAGATATCATCTATTGGTTTGACAAATCTGAATCGGTATTCAAGATGAGCAAGCCACACAAGGTTGCCGAGCTGTGGGGAGCTGCTACGGGTAATCCAGGAATGAACTATGACAAAATGTCAAGAGGACTTCGTTATTTCTACACAAACAATACATTGGAAAAGGTTAGTTGAGTCGCGGTTGAATTTTAGGATTAAAATGTGTGCGTTTTTGGTTAACTGCGCTACTTGCTTGATAAATTCATGTAAATTTGGATACCGTCTTCAACCTAGATATTTGTTAAATTTTTAGTAGTTTCTTTCTAATTCAAATTTAATAATTTTCCAGGTTCCAGGCACTAGT

KI verification primers: F19F10.1-NKIF: 5'-CAAAACTGGTGTAGACCGACAAATAT-3' F19F10.1-NKIR: 5'-TCATGGAAAAATCTAGGAACGAATG-3'

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Strain name: SYS455

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; F19F10.1 (devKi115 [mNeonGreen::F19F10.1]) V

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA