HLH-25

WormBase ID: WBGene00001964

WormBase description: Is predicted to have DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is expressed in several structures, including MSpa; anal depressor muscle; body wall musculature; intestinal cell; and neurons.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TTCCGTTCAGAAGCTGATTT

sgRNA2: ATTGAAGACTGAATAACTTT

5'HR: GCATGCAAGCTGTTCTAGAAATGTTTTAAAAAACAATATCCAAGTTTAAAAAAATCTCAATTTATTTTTAATTTACGAGGTTTCCATCTCAGCGTGTGACACTTTTCTTATAACTTGGCACCTACATGTATCCGGTGTGAATTTTTTCTGTTGCTGTGTGAGCATTTCCAGCAAACCGGAGTTCTCAAAATCAGTAAGGTTATATGTTAAAATTAAACTGGCTTATTATTTTCAGAAAATGAAAAACTGCCATATTTGAGTGATAAACTGAATGAATCCGTAAAATTTGGTAGTTGTATGTTCTATGCATTATCATATACATCAATCTGGTAATTTTTATTAGAATCAGATCTTGAATATTGGAAACTTTGCGAATTAAAGACAAATAATGATTAGGCAAGCTGATTAAATGAGTGATAAAAATGGTTAATGTCAATTCACAGTCTCCGCCGAAATTTCCCAGGCTGTACCCAATCAATATCCAGTCGTGGCAAAACTGGAGATGTCCTGACTCGTTCTCATGAGAACCTCACTGATGAACCATGGGAACCCGTGATGGGGACTGTAAAAATAGAGATTTCAGAATAATTCCCATGGCTCCAAACGCTTGATACAAAGCTCCACCCTCTGAACAATAATGGAACTATAAAAGGTAACCATTTATTCATTGTTCATTTACTTTCTTAGTGGATCACTGGTCTGCTGGTTTAGTATACTTTTTGATAATTGTAACATCAGGAAAGTGTACTGATTTTGATCAATTACTTTTAAAGCATTCAGTGAACTGTATACTTCTAGAAAAACAGTACGAGTTATCTCACTAAACTAAATCAATTTGTGCTGCTTTCTTGGTCCCATTTATCTGTTTTGTCACAATTATTCCTTTTTAACGGCTCATCTTGAATTTTTATAAATTCTACATTTCTTATTGATTTTAAATTAATGAAAATTATTAAATTTCAGGGTACC

3'HR: CTGCAGATGCCAAAGGTCATCCAATCCTCAATGAGCGATTATCGTTCCGTCCCATACAATCAAACTCCAAAGTCGGCCTCCGAACGGAAAAGAAGAAATATTACAAACGAGTTGATTAACGAATGCAAGACGATTGTTCAAAAATCAGAAGAAGAACATATATCGTAAGTTTAAATACTCTGTTAGCTTAAAATAGTTATTAAACTAATTCATTTTTTAGACAAGAAGTAGTTCTCTTCCGAATTGTGAAACTTGTGACTGGAGTTAATCTGGAATCTAATTTCTCATCGAATGACCTCTCCGAATCTACAAGACGGAAGTTTGATACTGAATCCGAAAGAAGAAAAGTGAAGACTGAACGTGAAAAGATTCGAAGAAAGAAACAAGATGACTGTTATGCTGAATTGAAATTCTTCATTTTGAACAAACAAATGGGAAGTTACGAGCAACGGTAAGAGTTTCATTATGGCTAATAAAAATTCAAAAAATTATTTTCAGATTAAAACTTGAACGGATCACAATTCTAGAAATCATCATTGATTACATTAAACACAATTCCGATCTTCTGTATCCAGAAACAATTCCCCAAATACTTCCTCTTTTAGCTGGAAAATCAACTGCTACTTGTGAGAACAAAGAGAATGAAAAGCCAAAAACACGAATGGAAGTGAAGGATCTCTTCCCAAGATTGACGTTTCAAGAAGTTCAAGAATCCCCAACATCTACTTCTCCATTGCTCACATTTCCATGTATTCCAATGATCCCAACTACTCAGTTCAATGTCTTGTCAAACTACAACACCGTTCCATCAATATTTTCTGCTCCCCTCCGATTCATTCTTCCATCACTTCAAATCTTAACACCAGAAACCAGTGACGAGGAAGAAAATGAAGAAACCCTCGATATAATTAATTAGTCTATCGATCTCCTGCAAATTTACAATTTCTGAAAAAAAACTTATTACTAGT

KI verification primers: HLH-25-NKIF: 5'-TTGGTAAAATCGGAGCTCGATG-3' HLH-25-NKIR: 5'-AAACCAGGAAAGTTTTGACAGCC-3'

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Strain name: SYS479

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; hlh-25 (devKi131 [mNeonGreen::hlh-25]) II

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA