KLF-3

WormBase ID: WBGene00003480

WormBase description: Is an ortholog of human KLF2 (Kruppel like factor 2). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is involved in fatty acid homeostasis. Is expressed in germ line and vulva. Is used to study obesity. Human ortholog(s) of this gene are implicated in several diseases, including chromosome 15q13.3 microdeletion syndrome; maturity-onset diabetes of the young type 7; and prostate cancer.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: AAAGCATCTAGATTGTGCTA

sgRNA2: CATGTGAAGACTCAAATGGT

5'HR: GGATCCACAAAAGAATACAATCACAGATAATCAATCAAGTTATCTTTTTGGTGGGAAGTCAATGATGATGAGAGAAAACAACGTTTATTGTTGAATAAAAAATACTTAGAAGTTCTAACAAATTATATACTTCAAGGGATTTCATGATTTTTTATATTGTAAAAAGTTGTATATTGTAAAAAAAGAGAAAATACGGTACCCTTCTGATAACTTTTTCTAAATTTTAAGAACTATGTACATTTATTCAAGTTTGCATATGAAACTCTACCTCCCAGTTCGGAATGTAATCAAAAAATGAAGAATTTTGTAGATCTACCATCTCAGGGTCACACTTCATGTTGCATTATTTATCACCCCCGCGCAACAAACAACACAGCATTGCTGCTTGTCATCACCACGCTTCTTTTTTCATCTTCTTCAATCATCTCTGATCATCAATGAATTCGATTACAGACTACCGCACAACGGCGAACTCGATTCAACTCGCTCTTCACCCTCTTCCACTACATCAAGCGAGCGATCGCCACTGCAGCGGAAGTCACGTATTGAATCAAACAAAAGGAATCCAACTGACAAGAAGTTTGTTGTTCATGCATGCACGTATCCTGGATGCTTTAAAAAGTAAGTAGGATTACTGTATTTTGGTCATGAAATCAATAATTTATAGATACTCTAAGTCTTCACATTTGAAAGCTCATGAGCGGACTCACTCTGGTGAGAAACCTTTTGTGTGCAAATGGCAAAACTGTTCTTGGAAGTTCGCACGAAGTGATGAATTGACAAGGTAAATTGATTGATAGATTCTTCAAATTCAACGTTCAAAATATTTCAGACATATGCGAAAACATACTGGAGACAAGCCATTCCGCTGCAGCCTCTGTGACCGTAATTTTGCTCGTTCTGACCACTTGAGTCTTCACATGAAGCGCCATAGCACAATCACC GGT

3'HR: CTGCAGATGCTTTAATCCGAATAAGATCACTACGAATTGCTTTAATTTTTATTTATACCTTATCCATTCTCATCATCTCCTTGATTCCCATCCTCTTTTGCCCACGTCACCAATTATCTGTGTTATCTCTTACCTTCTCATTTCAAAAAAATACTGGTTTTATTTGTTCACTTTCTCAAACACGTTTAATTTTTCCAATTTGCTCTACTGTCCTAAATTGAAATTTTTCGAAAAATGAATAAATTCAAATCGAAAATCGTTTATTTCACAAAATGGAGACCAAGCAGTCTATGGACTACATTATCAAGGGACCTGGAGGAACAACAAAGCCAATTGCTCCAAAATTGATTACTGTAAGATTTTGGATAAAGAAGTTTGGAAATCAAGTGAATTATGTTTCAGGTTAACCCATTGAATATTATTATCGAGTCTAATTCAGGACAAATTGATAAAACTCTGGAACTCAAGTAAGTGGCGATTTCCAGACGTGTATTTGCAAATGTAGAAATCGAACGAGAAATGTGATACAATTTCCCGAAATGCGCTCTTGAAATGAATTTAATTGGATGGGTCTCGCCACGAGAAAAATTATTGCAACGATTTTGATCTTTCGGACTTGTCATGAATTATTGTTCATGAAATTTAACAAAATCCCAGCCAAGGAAAAAAGAGCTTCTTCTTCTCGCGAGACTACTTTGAGTTTAGAAAATCTATAATGTTTCAGAAACATCTATCCATGTCGTATTGCTTTCCGTGTTCGAACAAATGGACCGACTAGATACACAGTTTGCCCCAGTAAAGGATTCTTGAGTAATGGCGAAAAAGTGGCGATTCAAATCACATTGATTGATGGAACAAAATATCAATCAAATCATCAGTTTATCGTTCAAGCAATGCCTTCTCCCGGAGACTTTGCTGATCGGAAGTTCATCTGGAAGCAACCAAATTTTCTTGGAATTTTTACAAATACTCACTAGT

KI verification primers: KLF-3-CKIF: 5'-ACAGTTTCCATGTTTGTTGACATAATAC-3' KLF-3-CKIR: 5'-TGAGCCGGTCTCCTAAAAGATG-3'

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Strain name: SYS512

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; klf-3 (devKi149 [klf-3::mNeonGreen]) II

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA