Single-Cell Protein Expression Atlas of Transcription Factors

WormBase ID: WBGene00000436

WormBase description: Is an ortholog of human MNX1 (motor neuron and pancreas homeobox 1). Is predicted to have DNA binding activity. Is expressed in VB neuron; ventral nerve cord; and vulval muscle.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: ATTGAATTGGAGTTGAAGAT

sgRNA2: ATAAAAAGATTATCAAGAAG

5'HR: GCATGCGTAAATATGCCTACCCGTTTGCCTACCCGTACATAACGGTAAACAAATAGATATGCTTGCATTGAAGCCTTTGAAGCTCAAGAAACGAGAAAATATACGTCCACCAAAAATACTTATTTTACAATTTTTACTCTAATTATCTTTCAATTATTTGAACTTTATGGGGATTTAAACTTTGACGAATACTTTATAGTTCTCAAAGAGTCCACATTTATCGAACACTTTGACAAAAAATTTCGAAAAAACCTGAAAAAAAATTTAGAGTGGCCGATGGTCCGCTGTTTTAAAAAATTTTTAGAGGGTATATTTTGACGAGTTTCGCATTGGCGCTAGGGCTGTGCGGCTGATGACTCGGCTGATGGAGACAATCAAGTTTTTCAACGTGTTAGCACTGATCAATTAAAGAATATTTGTATTTGCAGTATTGGCTGAGTCATGAGCTTCTTATTCTAGAAAAAAGTTTTTTTTTAAGTGCACACACCTTGACAAAACAGTCGGCTTTTTGAATCATCCGAGTAACTCGGCTGTCGGCTGTCGGCTGATTGGAAAAACGAGTTTTTCTGGAAGGGGTGTGCACTTTTTTAAAAACTTTTTTCTAGAATTAGAAGCTCAAGACTCAGCCGATATGGCAAATACAAGTATTCCCTAATTGATCATTGCTAATACGTTGAAAAACGTTGATTTGCTCCATCAGCCGAGTCATCAGCCGCACAGCCCTAATTGGCGCCACTTCACTTTTAATCAACAGAATATTCAACAAATGTGTTCCTACGAGAATGAAAATTGTGTAAAAATTACTTAAGTAAATTTCCATTGTATAAAATCATTATTTATTTTTTTCTAATTTCTTGTTTCTAAATCATAATGATTTCAGATCAAAATCTGGTTCCAAAATCGTCGAATGAAAAACAAACGTTGTCCATCCTCAACTCCAATTCAATCAACTTCCTCTTCTGGTACC

3'HR: CTGCAGTAATCTTTTTATATTTGATCACTTTTACTTAATAAATTCTTAATCAAAAGACAATATTCATTTCTAATTTATCTTCTTCCGCATCATTAAAACCTGTCAAATGATTACGTGATTTCGGTTCACTGATAACAAAAAATCGGATATTCTTCATCTCTCTCTCTCTCTCACCCCCAGGCTTCTTCTTCTTCTTCTGTCTTCTTTTTTATACAAAAAAAAAACACGTTACCTGACAATGCTTTAAATAGTGAAAATCTAGTGAAAACTCGCTTTTCAAGGGTATTTATGTTCGAATGAAAATGACTCCGCCCGTCCGAATACTTCCCATTATACAGTCACTTTGCTGATTGATGGGGTATCTTTTTTCGAGTTATATACGTTTTACTGCATTCACTTTTATTAGTATATTCAATTATACTATACATTCATACGTTCTTATCAAAATTACGAATTTTGTTATGATATCTAGCAGTTTTCTATTTTATTAGAAATCTTAATCTTCTCTAAAGGGAGAATCATAATACACCGTATTTCCTCTAATAGTTTTTTCATCTTATACCTTTTAATGGTTAAATCTCGGTTCAGTTTGAAGATATCAATAAATAATCAACTACAAAGAATTAGAAATAGAAATACAATTTTTTTACCGTTGACATTTTTTGTATTGGACACACGATATGTAAAATGTCAAAGTTGAACAATGAGGTGCAATAACAGGAAGTACGGTATATCAAACCATTTCGAAAAACAACATGTACCTTTAAAGCCGGATTTCACATCTTCACGAAATGATTATATTGGTATATTTTAATTTAGAAGTTTCAGTAATTATGTCAACCACAAGTACAGTACTCTTATTCATAACTTCGGTATGTGGTGTGGCTAACTAGT

KI verification primers: CEH-12-CKIF: 5'-CTTTCAGAGACTCAGGTAATTTCAATAGA-3' CEH-12-CKIR: 5'-TGAAGGATTCAATTTCATCAAGTGA-3'

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Strain name: SYS629

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-12 (devKi186 [ceh-12::mNeonGreen]) I

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA