CEH-28

WormBase ID: WBGene00000450

WormBase description: Is an ortholog of human NKX2-6 (NK2 homeobox 6). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in positive regulation of transcription by RNA polymerase II and regulation of synapse organization. Is expressed in several structures, including ABalaappa; ABalaappaa; ABalaappap; ABalapapa; and M4 neuron. Human ortholog(s) of this gene are implicated in double outlet right ventricle and spastic ataxia 8.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GAAGGAACTGCAGATTGCTG

sgRNA2: AAATCTGCGTAGATTGCATT

5'HR: GCATGCATTTTCTTCCTAGTATAAGAAAAAGAGAGCTAATAACTTTTTGAGAACTGTGAGAAAACCAAAACCAAGTGCGCATTTTTAAAAACTTTATGCAAATAATGAATTTAACAAATTTGAACTAGCTTAAAACTTACCATTTTCAAAGTTTTCCAAGTTCACTAAATTTGAATGGATGTGTGTAAAAATCTAGGAGTACAAATGTCGAGCATAGATGCATTCAAATAGTAACCAAGGCATTCGACTCTTCATGTTTGTTTCTGTTTTTAGAGAATTTTTTTAAAACGTATATTTTTAATCTATGGAAATCTTGAAAATAAAACAGAAGTCAACGTAAGATTTTCAGGCGAAAGTTGTATACATAATATTGTACAAAATCAGTTGCAAACTCTTCTACATATATGATTAACCATACATATCATATTTATGTTGACAAGCGTGGTTCAAGCTAAAAACTGACTAGTGAGATATTGAAAAATCAGATAAAATATACATAGAACCCCATGTGTTATTACTCTGTTTGCAGTATTAATTATTTGAAAAAATAACATTGTAAGCTATCAGCAGATTTTTGTTTAATTCAGGATTTATTAACAGTTGCATAATTTCCAGTGAATGTTGTGATACTTGAATTTTCCGACAAGTCAATTCAAAAGCTGCAAAAATCGTTTGTTGCAGTCATTTTTCTTATAATTTAAAAATAAATAATCGTGCAGTTTTATATCTGTCTGGGTGACCCCTCTGTATTTAATTTCTTTCCAAATAAGTGCACACCAAGATTCCAACGAGCCACAAGTGGTTGAGCAGATGGAGGGAAAGTGTCAAATTCTTCTAATGGATAACAAACAGTTAGTGGATGATTTGGTGTGAAAATCCCTCCTATTTTCCTACTCGTGATGACACTTCTCAAATATCAAAACTTCTAGAGAGAACTTTTTCACAATTTGAAGTTAGTGGTCCCAGGTACC

3'HR: CTGCAGATGCAATCTACGCAGATTTCATCTACTACTGTTATTTTACCAAGTGAAGTGATCCAACCGCCACAACAGTCCGCCGTTCCTTCAGAGTTTAAAAATACACTTCCGTCGCGTTTAAATCTATTTGAAGGTTTTGATCAGTCATTTTCTGAACTTACAAATCCATATCAACCAGTTATTCCGTTGATGCAGAGAGAATCCTTGTTAGGTGCAAGTTCATATTATAGTGAGTTCAATCGATATTTATTTAAAATAAAAGCTTGTTTGAAAAACCATTTGATGATTTCAGGTTCTCCAAGTCAAAATCAGAGATCTTATCAGAATCACCGTCAACATTCTAATCCAGATACTATCAATCTGAGATCACAACAGCAGAAGAGAAAACCGAGAGTTTTGTTCACACAACATCAGGTGTGTGGGTTATGATTTCCGGTTCAGGAACTTTGGGCTCTTCAGTAGTTAAAAATTTTGAACCACAGTAGTAACATTTAGTTTCAACACAACCAATTGTCTCCTTAGACGGTCTGGAAAATGTGAAAAAAACAACCAAAACTGACTATATACTGACTAATTTTTTCCATCAAATTCTTAAAAATCTCTGGAAAAGACACACGAGAGCATATCTAGATGAATCTTACGGCAGTCACCAACCAATTTTATCTTAAATCAGTTTAAAAAAAATTTACTTGAATGATTTCATTCTAGAATTTATTCTTAGAGAATGCAAAAATACGAATTAAGTAAGATGCATCATGATAGTCTATGTTTTTGTATACTTCACATGAGTTGTTTCATTTTCAACTTCACATCCAATGTCTACATTTTTAGGTTAATGAACTTGAAGAACGATTTAAAAAGCAACGTTATGTGACAGCCACTGAGCGAGAAGAGCTAGCCCAATGTCTTGGCCTCACAGCCACACAAGTAATGTAATCGTATTACTGTATGTTTTACATAATCATTACTAGT

KI verification primers: CEH-28-NKIF: 5'-TTTGAAACGGTGTTCGATCCA-3' CEH-28-NKIR: 5'-ATTAAACTCACTGGATTGAAAGGGA-3' CEH-28-NKIF2: 5'-GAAAATTGCTGTTTCTCATTCCAAC-3' CEH-28-NKIR2: 5'-AAAGCTGTGTTTATTCAGGTCAAGC-3'

Download

Strain name: SYS635

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-28 (devKi192 [mNeonGreen::ceh-28]) X

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA