CEH-23

WormBase ID: WBGene00000446

WormBase description: Is an ortholog of several human genes including DLX2 (distal-less homeobox 2); DLX3 (distal-less homeobox 3); and NANOG (Nanog homeobox). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in determination of adult lifespan and positive regulation of transcription from RNA polymerase II promoter involved in neuron differentiation. Localizes to nucleus. Is expressed in intestine; nervous system; and tail. Human ortholog(s) of this gene are implicated in amelogenesis imperfecta type 4; orofacial cleft 15; and split hand-foot malformation 1 with sensorineural hearing loss.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GTTAGAGATGATGAAGACAC

sgRNA2: GAAACTGGCAAAGTTTGGAA

5'HR: GCATGCTTCCAAACATTGAGACTTCCTTGAAAATGACAAATAATTGGCTTCCATTTGCATGTAAAATATGTATAACGTTCGATCATATTTAAACTTTAAAGCACATTAGCCTGTGAGCTTTGATATCTGACTAACAGAAATATGTTAGACACAAAATTCGATTGCTCTCAATTTTATATTTGAAACTTTCGAAATTTTCACCAATTAAAAATTTTACACTTAAAACTCAAATTGTTGAAAATAATCTAATAGGTTGTAGTAAATTGGAGCAGCTGCAGAAATCGAGAAGGAAAATTAAGTTTCAGATTTTTATTTTAAATTCTCACTTTTTCATTTCTGATATGCAAAAATGTGATTAAATAAAATCCTCCAGACATTCATTATCTACGTCTTCCCATCTGATTCCATTCATCTTTCATAATCCGATTAGCAATTTCAACATTTCCTGGAAACACTGATCTTTAAAAATTAATCCTTTTCATTTTTGTTTCGTCTGACTCATTTAATAATATCTACACTGATATACCTGCACAACACACTGACTACTATTATTACTTTTACTACTACTATGACTACTATAATCTACTAATGAGTGGTAAATTTTGAGCTTTGATCATTCGTTCACATCCCTTGGTAATTGGAGAGAAGCAGTGTATTTTCATATTCCCAGACTCACACGTTTTTGAATTTGTCTGCAATAGTGAAAACTTTAGTTTCTATAATTTACCATTCAGGGGTATCAGCATTTTATTGCACCCAGTAATTTTAGATAATTTATGGTTTGAATCTGTTTTTGTCCAACTTGATCTGATAAACTTAGAACAGTAAGTTCTACAATTGCCAAACTTTTAATTTTTTACCTCTTTACATTTTTCAACATTTTTACATTTTTTGATTAATCTTTATTTTCTAGGGTACC

3'HR: CTGCAGATGGACACCCATCTTCCATTCCAGACCTTGCCGGTCTCAACGCCGCTGCCTGTCTCCTCGTCATCCCTAACAGGTAACATCAAATCCATTTTGAAATGTGCTCTTACACAAAGCTCCTTAATATCCAAATCGCATTCCCCCACACCATTTAATGATTTCATGACTGAAAACCATCTGTCAAACACGACAATCATCCGATTTTCATTGCTGATAAGCTGATTTCGGGAATGATGATGATAAGATTTGTTAGACTTATTCAAAAATCGAGAAAGATGAGAAGCTGTATGGCTGACATATCATTTTATGAATTCAGACGTTCTTCAAACAATTGCTGCTTTACAAGCCTGTCCCACATCCTGTATACCTTCAACATCAACTGGAATGCTTTCACCGAATCTTCCCTTCTCGGCTACAATTCCACGTGTCAACTTGTTCCCACCATCACAACCAGCCAATTCTTTAATACTGCCAACAATTCCAGCACAGGTGAATGATTTTCGTATTTTCAAAAAACCTCTTAACCCCTTTTGTATTTTCAGCCTTTTATTCCAAATCCATCTCTCCTGCAAGCCAATCCATCTGCTGTCGAAGCTCTTGCAAATGCACTTTTTGCAACAACAAGCCGTCGGGCTTCTTGCCCAGAACCTCCTGCATCCTCTCAAGCAACCGTCACGCTTCAAGTCCCATCTACGGGTTCTCCAGAACGACGACGTTATTCAGAAACAAATATGGAGGTTCTGCTACGTGAACAACTTGCACAATTGATGCCTCCAACCTCTCAACTACCCGGAATGCCTGGATGTTATTATCAACATGTTCCAGCTGCTGGAACTTCAGGAATTCAAGGATCATTAGATGCGGCGTTAATGGGAGCTGTTCCCTTGGCTATGAATAGTATGGCACATAGTCGGAGAGCAGCAAATCATAGAAAAGCAAGGTAAGGAGAATAAACCAGCAAAAACTAGT

KI verification primers: CEH-23-NKIF: 5'-TTAAGAGCAATTTATGTTCATGGCT-3' CEH-23-NKIR: 5'-AAGCCAGTATACAAGTATATTGTGCTCT-3'

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Strain name: SYS636

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ceh-23 (devKi193 [mNeonGreen::ceh-23]) III

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA