PES-1

WormBase ID: WBGene00003976

WormBase description: Is an ortholog of human FOXL2 (forkhead box L2). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is involved in embryo development and nematode larval development. Is expressed in several structures, including ABplaaaap; ABplaaapa; body wall muscle cell from D lineage; neurons; and somatic gonad precursor. Human ortholog(s) of this gene are implicated in blepharophimosis, ptosis, and epicanthus inversus syndrome and premature ovarian failure.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: TTGGAAAGCCAGGAACCATG

sgRNA2: ACTTTTAGGAAAATGAAAGG

5'HR: GCATGCTCCCTGACACCGACTTTCAGATTTATTTATTTCTGTAACTACAAATTTCAGGTAATTCAAAAATGAAGGGCTTCAACATCTCGGACTTGTGCCTCGACTTGGACAGCTCAACTTCCAGCTCTTGCTCCGTTTCACCAGGTTAATTTTTCTACTGTTTCAAACAGTTTCAAACAACGCTTAAAATGTGCGCATATGTAATTTAATATTTGTTCCAGCTTCTTCCTTCCACACCCGCTCTGAATCAGTCGGTCAACAACAGTCTGGTAGAAATTCACCAGGTAAGGCAAAATATTTTGTTTTTAAAATTGTCAAGTGTTTCACTACTATGATAGTTTCATACATTCAGCAGATTTTTAATTACTATGTTTACCAAAAAATTTACATATCTTTATAATTTTTAGTCTCAAGCTCCACCGAATCACCAACCAAAAGACCAAAATACTCGTACAATGCATTGATCGCCATGGCTATTCAGAGCAGTCCATTCAAAAGTTTGAGAGTCTCCGAGATCTACAAATATATTTCTTCCAACTTCTCCTACTACAAAAACCAGAAGCCACTTCAATGGCAAAACTCGGTTCGCCACAACTTGTCTTTGCATAAAGAGTTCCGAAAGGTTCGAACTTTGGATGGAAAAGGTTTGTGTTTTAACAGGACTTAATAGAATAATAGTGTTTCTTTTCAGGCAGCTACTGGGCAATGACTGCGGATTTAGGAACTGATGTTTATATCAGCAACAACTGCGGAAAGCTTCGTCGCCAAAAAAGCAAAGTGGCCAAGTTTCCACCAATGCAACAACATTTTCCAATCCCTCAGCTTCCAACCCAAAACATTCACCAACTTTGTATGCAAAATCCTCAAATTCTTGCTACATTGCTTCAAAATATGTATCTTCAAAATATGCAAAACCTCCAGAATATTCCCATGGTTCCTGGATTTCCAATAATTCCAGTTCCCATTAATCCTACTTCCTTCCATTTTCCTAAAAGTTCTGGTACC

3'HR: CTGCAGTAGAATAGCCTTCTGAGTTTTTTGTTAAATTGATTGAAATTAACTGTTGTCCAAAATCAGTTTTTTTGTTTTTTCCCCGATTTGTGTTTAAATTATTTGATTTTGTTGGATTTTGATAAATATTTAGAAAACTAGATTCTCTGTATAAAGCTTGAACTCACCGCAACAATTAGTGAATTAAAGTGCTCCAAAACAAGAAGACAAAGTTAGTGATTTGCATTAATTGAAGGGTTTCCTTCTCTTTTGAACTCCGTGGTTTGTGCAAAACTACTTATTTTCTTCCGCTTGTAGTTTAGAATGCACCCGGGTCAAAATAAAAAGTCTGAGTATATCTTCCGGATTTAATTTTTTTTCAAATGTTTCATTTACAGTGATTTTTCCAATAAACAATAATCTGTAACTGTGCTTTAAAAATTTTCAATCCGTGTATAGATATTTCTCATTCAAAAAGAAATTACCAAGAAGCAGAAACATTCTACTTTGAAGTATTATAATAATTCAAGGAACCAAATTATGCATTTTTTTAAACTTTAGCCAAGGATTTTTAATACATCGAATTGTTTCATATATGATTCACTTCAAGCTGACAAAAACTAAAAAAAATGGCAAAGGTTTCAATGTACCTTTTCAAAAACTTTTAAAGTGTTTAGCTTGTTTGTTTTCACAATACAATACTACACAAAAGATTTTCCGAAATACAGAAGATCTAAAAAAAAACAAAATGGAAACCGAAAAAATGTTTTTTAATTATTTTGGGAAATTCACACGATCTAAAATAATTATTTTTTAAAGCCAATTTACATAAATAAATAAATAAAAAACCTTCAGTGTGCAATATCCTTAAAAGTAACTCAAAATCATGAGGGAAATTACTAGT

KI verification primers: PES-1-CKIF: 5'-CTGTGTGTGTTCTCTCATGAATTGC-3' PES-1-CKIR: 5'-GAGGACATAAAAACGAGGAAAAACA-3'

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Strain name: SYS646

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; pes-1 (devKi203 [pes-1::mNeonGreen]) IV

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ZIP-8