Single-Cell Protein Expression Atlas of Transcription Factors

WormBase ID: WBGene00008092

WormBase description: Is an ortholog of human GMEB1 (glucocorticoid modulatory element binding protein 1). Is predicted to have DNA binding activity and transcription coactivator activity.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TCACTGGTTGATCCGAACTC

sgRNA2: GATGAAAAACGAAGAGATGC

5'HR: GCATGCAAAAATATATCTAAAAATTAATTCCAGATGTTTCTCCACTGCACATGAAAAAAAACAAGCAAACAGTTAATTTTCAAAACAAAAAAAAACATTTTAAATGTAAAAATTGTAGCTAGCTCCAGGCTCCAGTCATATGACGAATGAGGTAATCGTTGTTCGTTAAAAAGTATAAAATCACCTTCATTATCGGGCCTCTCATGGGTATTTAGATCATTGAATTGCGGTTTCAAAAAGTAAAAGTACAAGTCCGGGTCACCCACCACTTTTAATGGAATGTGGATATGCTCAAATACAGTGCTCATTGTAATGGGATGGCGTGGAGGATGATTTACCCTGTGTAACAGAAAAATAGTAAAAATGTTTAACACCGGATGTTTCAGAAAAAATCCCATTTCAAATAATTTTGCAGAGCATTTTTTTGAATTCTGTGTAATTCTTCACAACAAACGTACTGGAAAAAAATCTGGAAATTTTTGAATTTAGTTGGGTAAACAATTTTAGAAGCTGATTTTTTTTTCAAAAAAAAAGCTTCCATGCAAATTACCGGGCCTCGCAAGAACTTAAAAAAACTCTTATGAAAAACATTTTTTTGCTAGTTCAAAATGCTCTTCCGAAACTAATTTTTCTGCAGTCATCTATTTTAATAATCATATTGTTTGTCCCTAGTAAGGCTTACGATACCACAATACTTTTTGGATTTTTCTCTGCAATTTTTCCAGTATAAATGATATTTTTGATCATTGAAATGCCTTAACTTTTCAAAAAAAAAATCATTAATTTATTTTAAAAATATTCAAAAACACGCGAGAAGCCAACAGATCCAAGCTGGCGGAAAACTAGTTGCGTGTCAGAAATTATCGTGTCGCGTCGGGTCGCCCATCTTTATTCATCGATTACCTGAAAAGGTACC

3'HR: CTGCAGATGTGTGACGACGATGATTTCGAATTCTCTACTCAACCCGAATTCGGATCGACTAGCGAGATGAAAAACGAAGAAATGCTCGTTCCAAGTGATCCAAATTTCGAGATAGACGGGAGACGCGTTCAGGAGAATATGTATGATGTGAGTTGGGATGTGAAATTTTGAGACAATTTTTTTGGAAACAATTCTTCCAAAAATTGAAATAGTCTTGTTAGTCTCGGAAATACTTTTTTGGCTCGAAAAAAATCTTCAATTTAGTTTGAAAACTTCAAATGCACCTACATTGCTGTCTGAGATACGAAAAATGCCATTTTGGCTCCAATTAGCAATTCGAAAATAACAAATAAATATTACAGCAAGCATCGGAACTCAGCTATACTTACCTGGATACTCCATCTGAACATATCTGGACACCAATTGTACCTCTAAATGCCACCACGGTGCCGGTTACCTGTGGATATGTCAAAGGAATTATGCATCTCAAATTGTTCAGGTGCCCCGGAATTCGTCAGGCTTGCATCGAATATGAATGTATGGTGGCTTTTATAATGTGAGAGAATGAATTTATTCTGGAATTTCAGCACAGTATCTGACTCCGAAACAATTTACGGAACTTGGTGGAAAAGGGCGACAGAAAGACTGGAAGGCGTGTATTCGTGTGGAGAATGTTAGCTTGAGGTGAGTGCAGAAAGATCAATAAATTGTATGCTTTGGTTTAGGCTCAGGCGTATGCTTAGGCTTAGGCCTCGGTTTTAGGTTATGTGCTAGGCTTAGGTTTGGGCTTAATCGTAACCTTTTGCTTGGGACTGCTAGCCACGTTTAATTCTTAACGTTCCAGTTTTAATTCATTAAATTTCAGAACACTATTGGAGAACAAAACCATCGATTTCTATAATCACAAGAGTATCTGTCATGGACAGTGCCAAAGTCGCAACTACATGAACTCAACGGGCAGAGCTCCAGTTAACAAACGTCACCTCGAAGATGTTCTAACTAGT

KI verification primers: GMEB-3-NKIF: 5'-AATAGGTATTCCCCTATTCACCCAT-3' GMEB-3-NKIR: 5'-TTTGCTCCGTTGGCTCAGAT-3'

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Strain name: SYS647

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; gmeb-3 (devKi204 [mNeonGreen::gmeb-3]) III

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA