KI information : 	mNeonGreen knockin at C-terminus
sgRNA1 : 	TGGAGAGGATGAATTGCCAA
sgRNA2 : 	"ATTCTGCTCACTGTTCAAAA
NHR-49-CKIsg3: ATGGAATCTTGTGATTGGAG
NHR-49-CKIsg4: GTTGCTGAGAGCATTCCAGT"
KI-homologous recombination fragment-5'arm : 	GCATGCTCTACTGAAAAACTATGCACCACAACATCTCATTTTGATGCCAGCATTTCGAAGTCCCGATACAACTCGAGTTTGTCTCTTCAACAATACTTATATGACACGTGACAATAACACTGACCTCAATGGATTTGCTGCATTCAAAACAAGCAATATAACACCCAGAGTTCTTGATGAAATTGTCTGGCCAATGCGACAACTTCAGATGAGAGAACAAGAATTTGTATGTTTGAAAGCACTTGCATTCTTGCACCCAGAAGCCAAAGGACTCTCGAATAGCTCACAGATTATGATTCGTGATGCTAGAAATCGTGTTTTGAAAGCTCTTTATGCATTCATTCTCGATCAGATGCCAGATGACGCACCCACAAGATATGGAAATATTTTGTTGTTGGCACCGGCTCTCAAGGCTCTGACTCAGCTGCTCATTGAGAATATGACACTTACAAAGTTCTTCGGATTGGCAGAGGTATGAAAGCTTTTCTTGTTTGAATCCGTACATAAATGATAGCTTCAGGTGGATTCTCTGCTCTCCGAGTTCATTCTCGACGACATCAATGATCATTCGACGGCTCCGGTCTCTTTACAGCAACATCTTTCATCTCCGACAACGTTACCGACTAATGGGGTTTGTTTTTAATTATTTTATTAAACTTTTTATTCTAAAAAAATACAAAAATAGCGAAAACGGCCAATGATTTCAGGGAAAAACCGTAAAATTCTCTTTTTAAAAAAGATCTTCCATTTCAAAATTCATATTTTTCAGGTATCTCCGTTAAATCCAGCCGGATCAGTGGGAAGTGTTTCGTCTGTTTCTGGAATTACACCAACTGGAATGCTTTCGGCCACTCTTGCAGCTCCATTAGCCATCCACCCTCTTCAGTCACAAGATTCTATTTTAAACAGTGAGCAGAATAATCATATGCTCGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGTAATTCCATTTTTTCTCCCAAAACTCTTCACCTCATCTCATCTCCAGTTCTATTCCGAATCTGTGATCTTTCATCTTTTCCCTTATTGTTGTTTTGAATCATTACCCCCAATTTATAAAATAGTCCAGAGCCCAGCTATAATGTACATAACAAAAGAAGAATACCCTTCCGTCTCAGTAGTTGTTTCATCTTGAAACCTGTCTAGGCCATCTCTATATCATATCAGGTTATTGTTCTCGTCTCCCCCAACTCTTCTTCCTTAATCTTTTTTCCAGACGGGCTGGCTCTCGTTTATTCTTGTGATTATTATTGTCAATCAAATTATCAGTTTAGTACTTCAATATGTATAAGAAGTGACTGTCTTGGCATTTCCCATCTCTATTTAGAAGATTCCCTTCTTCCCATTTTCCTTCCTTCTTTTCAAGCGTTCGTACCAATTTATGTTTGCAAAAAGAAACTTTTATTTATACTTTTTCAAAGCTCATCGATAAAATACATTATTATACCATATTTTATTATTCGTATCAAATAAACACAATTGACTCATCATTATGAATTCACTCGAGAAGTTCAATGCCACGTCCTCCACCACCTCCACCAAGACCGTAAGTTTTATTGCAATTTAGAAATTTAGAAGAATTTACAAAAAGTATTGTTTTCCAAATGTTTGAGCACCTGGTGTGAGCTGCTGGAGGTGAAGCTGAGCTTTTTAGTTTTTTTTTTCAAAATTAGATTTTTTTAAATTGTTTATTTCTCTAAAACTCTTCAAATTGATGATTTTTGTTTTGATTTCAATTGACACGATCATCTAAATGATAGTGGATATATGACCATTTCCCAAGACACTTGAGATCAATTCAATGATCCGAACAGTGTACATAACGGGGTAAAAGTGATGATATGTCTATTGGAAGAGGGAAGATGAAGAAATTGTGAGCATTTAGGCTTAGGTACTAGT 
KI verification primers : 	NHR-49-CKIF: 5'-TATTGATGGTTGAATGGGCAAA-3' NHR-49-CKIR: 5'-AATGTCCAAAACTGGCTGAAGTG-3'