microRNA name: let-7

WormBase ID: WBGene00002285

WormBase description of let-7: Enables mRNA 3'-UTR binding activity. Is involved in several processes, including negative regulation of Ras protein signal transduction; negative regulation of gene expression; and regulation of development, heterochronic. Part of RISC complex. Is expressed in several structures, including gonad; hypodermis; somatic nervous system; touch receptor neurons; and vulval precursor cell. Is used to study cancer.

Conserved microRNA sequences of let-7:

D.melanogaster: dme-let-7, dme-miR-963, dme-miR-977, dme-miR-984

H.sapiens: hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-196a, hsa-miR-196b, hsa-miR-98

Location: Intergenic

Promoter length (bp): 1990

Promoter sequence: TTATCTTTTAATGTCTTCACaatttatagttatctcgttaattaagatgtttttttgccgatgcgggcacactttctatacatttttggaagctacaaaaaagttgagatattagctaaaaaatgaacgtttctttcaaatcataacttttttttgagaacagaacgtcttattttgaaatttggtagttttggaccatttgagttaaaaaagcaaagcccgaatttcggtaccctccctcttttaagcctgtaaaaatttagaggggggggggggaattttttgagatagttaaaccgttttttgaatgttaatttcatgaattttagaagcgtgaacctaaaaaaataccgcataattcattatttttgttaaaccatatgcctaataaattttatgtttttattttggcaacagcagcccatttgttaagtgaccccatatgcagcatcgatacagtttttttttaattggtggtcacttttttccgtctcaaaaaaaaactaggttatctttttttcacttttcaatctgtcctttcgaagatgttttgatatcgctttcgtaaaagatatgcagtttttttcctgaaaaatagaatagctggacaaactttaacttaacttacatgcccatttcaaatgtttctttcaaatttcccttcttcctttaccctcgaatgcacatgttgcaaaatccaggtcaccgcaataaaataccatcaaaagtggaacaaaagttccatttcttttttatccgattcgaaagagagagagagaaaaaaaaaacatctacgcgaatttaatcgggtgtcacgcagtgctagccgttgcacaggtcattgtttgcacggaaccaacttgcactgtttttttgtgggcggagttgaaagcgaaaaaaaactatctaggagggaactgggtcactcggatgtccacagtctgtccactttcaattcctcttcattcgtgcagccgttttttgctgtgacgtagtagtgggtatatctttctcgtgaaataacatgttgtgttcttcgagtgaatatattctttgaggcttttttaaggcagattttgaaacaatatagcaaaatttattgctctataatgaaactaaaaaattacaacttttaactacaaaaacatgcaaaaaaatggtataatttgaggtttacctaattttaattttgatttttcaaacatttaaacatccataaaaaaaatacaaaatcattttttggcatcatttctcctattttaaataattttattttaaaaattagcttctcaacattggaaaaaatgatcgaacatgcaacttttctagctataattgaaaatctgtcattgagcaattaatatagacccattttttcaacacccatacctttcttatcagtttccctcttgccatctttcgtgtacatttgcaacattttctggatcatcaatcaagtgtgcactgaccactctctctctcctcctatttccctccttctaaattcgtctaggcgtcgtttgcgtatgtgtatgtagcggcaggcactgccccgtttcacgcacttttgcctgataccatggaggacgacggcgcccggtactgccgcccgggcccgcggttcgcaacaatggagcatacggagacgaagagacgcagcgaaccgcgagccaccaagttgctccgtgtgccgccggcgcccttttagccccacctctcgcgggtttctgttcatatatgagaagcgcatcagtttttttttcttattctagatgagtagcccacctagcagcggtcgtttttttctcttttcgtaggaaaattttcagttacacttatttattagaaacctagatttagagacttttagaataactttacttcctagaacacatctccctttgaatttatatgtctaatttaacaacaagtactaatccatttttcaggcaagcaggcgattggtgGACGGTCTACACTGTGGATCCGG

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Tissue specificity (embryo): Hypodermis

Tissue specificity (L1): Intestine, Coelomocyte

Number of insertion lines: 4

All strain names: SYS1747, SYS1748, SYS1749, SYS1750

Representative strain name: SYS1749

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view