microRNA name: lin-4

WormBase ID: WBGene00002993

WormBase description of lin-4: Enables mRNA 3'-UTR binding activity. Is involved in several processes, including negative regulation of gene expression; oviposition; and positive regulation of vulval development. Part of polysome. Is expressed in several structures, including P5.p hermaphrodite; P7.p hermaphrodite; gonad; neurons; and ventral nerve cord.

Conserved microRNA sequences of lin-4:

D.melanogaster: dme-miR-125

H.sapiens: hsa-miR-125a-5p, hsa-miR-125b, hsa-miR-331-3p

Location: intron anti-sense

Promoter length (bp): 2409

Promoter sequence: CTATTAGTATTGCACCAGACtttgaatggttataactcggatcattctaaaagtatctacaattaattaattacagagctatagaaaaataactaacaaatattttatctgttgacaatttctgtataaagcaaacaaaaactaagctatattaaacaataaggtgaaatactaatagtgaaaatacggaattttggaatttggaacttgaagcattttctattctggttttagatcatattttctggttattgaaattggaatagaaaatgaacacattaaccgatgtgcaaaacaatttcaacactctaataaaaatttgaatctagagcattgccttttccctatttcaactgatatttcctcttccaatacctgagtgataaccgggaggttttactttcactattcacagcgccagtcgttcatgtcctgcccattccgtagtttgataataatcgaactgttcaccagcgccgttttccgcatatttttgcaagtgtcacttttcatgtacccatttgagggaaatgaaacaacgaaaaaccagttagacttctacagtttcttcttcttcttctccttctctttcactccaccaactaatcacctaccgtaccccttactcgttttcttgtgttctctcaaatttcaatattctatcacatggttcaggaggaaaatatttgtgtctgacgccacttgatcgcttttttgtctattttaaaaatgtagaaaatacatagaaactgtgggatttcacgaggtagttatgaaacgagatcgatagttttgagcgagaaaaggggtgttctctacgttcgattgtgtggaaccgagaatagttagacactatctagagagcattcaaaaataagagaatttaagatagggacgtgcatgacggacgcggcgccatcggccagaagaacactgccattggctttcatatccctttccttatcccctctttctgactgttgccattgaatatttgagctattttgtcaaatgacaaatccaaaccacctggctcattaaaattcaattaaatacaattaaattcaagagaaagatgtgccaggattcatatattcaaatttttatgtgcatgtaggtattaccgtgtcattctttctacgccattctttcaaagtaaactgataagcagtaatggcgcgagcaccgccacgccaaacgcccaatgtcatttcaaacatgtattgtttactagctgaagtcaacatggcaaagttttcaattttattcgtattttctatatgtgaaggtaggaaaaagtctaagcttcagaataggaaagctcaccatttttttaaattttgttgcaaaattttataggtcagaataaaacatacagtatcatattcttgattcagtgtttcccattaagccttgtttgaggatagttcaaagttcactactttcgaggattagaagtctcaaaatcatgatgtagaattgaaacaagtgttgccaactttcagcagaaattcaatttagtagagtacaccaactcttaccaatgttattgttaacaaccaatccaatgctcatcttctctgaaatttctctgaacacagttgttttagaactagataaagttctcagaagtttcccataggttactgtaacgacctttcccacccattatgtcttggtaccccttcctagagacggcgaatttcgaaaacgaatgtgtatgtgtaccagccgtccagttcaaatgggcacaggggacaatcaaaattgcacgacctctctctctctttctatgtttcgtttttcatcctcttctactccgcgctcactccgcaccgcttcttctccctaaacagccgccctgaaaggctagatactcagtgagagacaataaaagtcgacgagacgccgagtctcccttactgcaaaaatgagaggggagcgaggtggtctacgtctctgtggcacctaacactatttcggggacgcgtcgccaagcggtggtggtgccagcctcacggaaaggcttgcggggcgccgcgctcgtgtccctccgtgctctgctgcgtgtgccttccgcttctcgtctgtcctctctctcagtgcgcggggggaccgcggcaaaaaagaataacgacgaagcgaccgaatgacccagtctcttcacttctctactttcgatcctcctccttcagctactcctcccatgtccatccatcctccgccccatcactcccagagaccctttcggtcactctttccaatagactctaccacaatcggtcggactcatcacacttacctttcaaatatctaaactattcctgaatataataaatcttatagtttttagattctagacaatttctagagttttggttggtttatGAGTTTATGCTTCCGGCCTGT

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Tissue specificity (embryo):

Tissue specificity (L1): Pharynx

Number of insertion lines: 7

All strain names: SYS1781, SYS1782, SYS1783, SYS1784, SYS1785, SYS1786, SYS1787

Representative strain name: SYS1786

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view