microRNA name: miR-1018

WormBase ID: WBGene00050924

WormBase description of miR-1018: Is affected by several genes including eat-2prg-1; and meg-3 based on RNA-seq studies. Is affected by nicotine and multi-walled carbon nanotube based on quantitative PCR and RNA-seq studies.

Conserved microRNA sequences of miR-1018:

D.melanogaster: dme-bantam

H.sapiens: hsa-miR-450b-3p

Location: intron sense (ncRNA)

Promoter length (bp): 2000

Promoter sequence: TGGAGTTAAGCTCCACTATAATCTATAGCACACCTTCTCTTTTGATTACATACAAGTAGACATCCGAAATAATACAGGGTTGATGTCAATTCCCCTGAATACAGCCCTCGACACTGGGTAACAAGCGCCCGCCAAGACGCCCTGCGGTTTTTGAGTTTCAAATGAAATATATAAATTCGGTACATTTTTTGGAGCTTCCAATTGAAAAGATGGTCTTATTCTGAGGGCTGTATCTCGGTTCATTTGAAAGATACAGACACATTTTAAAATGCTAAAATATTGCAAAATCTTCGATCTACATTTTGGCGTACACAGTTTTAAAAGTCAGCTCAAAATGAGGTCGCCGCGTGGCGTTTAACACGGTATTAGAGTAAAGTCGATTAATTTCAAGTTGAGAGTACTTAGAATTTTGAAATTTTAAATATGTACTCTTTGAACAATTTACTTTTGAAATTTGAAAAAAATTTTGATGACGGTGTTACGTGGCAATTTTAACGAACGTGTAATATTCTAACACTAAAACGGGAAAAATGTTAATAAAACAAAAACGGTCCGAACCAGTAAGGTAAGAAAATTTACTTGGTTTTTGGAAATTTTTTTTTGTAAAATCAGGAAAATTTTCTTACAGGTCAGGTTTCTACAGCCCTCGTAATGCGAAACAACTATGATATGTGATTTTCTAAATTACGGAAAACTTATCGAATCGCAATTCACTAAAGCGGCAGGTATGCAAATGCTCACCGTTTTCTGAATATTTTGAAAACGACGGCATTTGTTTACAGAAGAGCAGTACATCTCTCTCGCGATTGCCAAAAAAGAAAGGTAGGCAAATGCTTCTCATTTTCAGAAAAAATTGAAAATTCGAGCAATTTGTTTACAGAATCGAATTTTGGAACTGTTAGCTGAAAATTGCCAACACACTGCAAGTGAGCAAATAATTCTTACTTTCAGAGAATTCAACTCGAAAGTGAGGCTTATTTGTTTACAGAATGTAAGCTTCTTGTCAGTGGCATCAAAATCTGTTCAGTGAGGTTGGCTACATAGAAAAAATTTTAAAAGAGATTGGCGGATTGAAAAAGCAGTTGACAAGCTTACTAATGAGAGCAAAAAAGCCTAACAATGCCCATTGTTAAGACCAGAACCTCGACATCAAAGTTATGACAAAAAGGTAAACACGATAATTTATTCTAAACTAATAAAAAACATTCTAGAACATTCCAAAGTTTTTCAAAAGTTTCCAGATGGTTCTAGAACATTCCAGATTTTTTTAAAAATTTTCTAAAGGGTTCTAGAACATTCTAGAGTTTTTAGACTTTCCAAAAGATTCTAGAACATTCCAAAATTTTTCAATAGTTCCCAGAAGGGTTTAGAACATTCCAGAAATGCAATAATTTTACAGAAAGCTCTAGAACAATCCATGTTTTTTTTAAAATATTTTCGAGAAGGTTCTATAAAATTCCAGGTGTTTTTAATTTTTCCAAAAGGTTTCAGAACATTCCAAAATGTTTCTAAAGTTTCCAGACGGTTATTGAGCATTCCAAAGTTTTTCAAAAGTTTTCAGAAAGTGTCAGAACACTCCAAAAAATTTTGAAATTTTCCAGAAAGTTTCAGAACATTCCACAGTTTTTCAAAAGTTTTTCAAGGGTTCATGTTCAACATTTCAATGCTTTTCAAAAGTTTCCAGAAAGTTCTAAAACATTCTAGAATTGCTTCAATTTCCCAGAAAGTATTAGAACACTTCAGAGTTTTTTTTAAATATTCCTAAAAGTACCGCTACAGTACCTTGACAACATTCTACACCCACTTCCAACCCAATATCTCTTCAAAAGCTCAAGGCATTTATTTTTTCAAACAACAGTAACTCAGTAGTCTTACAGTAACCCTACAGTTCTTTTTCAACTAAAACTTGGTTGAAAAGTATGTTAGGCGATTCAAAACTTTGTTCCAGAATCTCTATTTTGCCTACAGAGAATGTTGAATATCACGCTGATGTTGTAAAG


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 2

All strain names: SYS2165, SYS2240

Representative strain name: SYS2165

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage