microRNA name: miR-1022

WormBase ID: WBGene00077721

WormBase description of miR-1022: Is expressed in sensory neurons; uterus; and vulva.

Conserved microRNA sequences of miR-1022:

D.melanogaster: dme-bantam, dme-miR-306*

H.sapiens: hsa-miR-450b-3p

Location: Intergenic

Promoter length (bp): 2004

Promoter sequence: aagtggaaaagtaagtgtcctattttgattatatttgaaaactttaatttagtcagtcatggatcctagataccgtggaatcatgtataaccttactacaccggaaggcatggcttatcacttaagttactctgttagccaatatcaacaattgtcaagatgcttcactgagcagctaacattatatacttgctcacctcaattgcgtctgaagcatattgcagaagcgtcaatgtttatttcaaaaagcatccaacattcttctgactgtacgatgaaggtaatcataaaactgtctcgacagtttgcaacaacttctcaacctaccctttagaaattgaaatcaaagaagttgctaaagttagctgagaaggtgcggcgaattctcaaaattcaagacgtaatcaaaaaagcgcaactttttgtgcaaaagtaaatagtacgaaaattatattaacactctcgaaaaagtgtacaaatgttatatacatttatgtataacagtttccaacattcagccatttcactaaagctttgacagaaattttcgtacatttttaattttttgatacgattttctataattttagtgtatttagacaatgttcagacaacgtttcaaggcatagctagaatttttttttgttaatatagtttagacgtttagaataaaaacaatatcaacaaataagtcgacaaaaatttttgcagttttatttcatgaaattacgtgtttaagggtaatgtgtttcaatacagtggatgtttatcaaaacactatgacttgaatcagaacaacactctaaaagagttggaatcgctacacgaacaccgctatgacgtggtatcttgcgtagacaaacatctggaaacaatatacgagtgttgtgacgaaaatcgtagaaacatatcagaagctcttcgtaccaccgcacttctctgggaaatgtcacaatatgacaatgctcactttccgaaaatccgtcaatagtcttcaattgtttttggtgaaaactcgtttggagcataataaacatcaatttattttttttcaatttaagtttacacatacacaagtaaacatctacctaatctatatctttcggaagtttgaaaggggccccgaaattaggtctctccaattaagttgtttcaagttgtactactatagttattaaatgtgagcttttcaattaattaagctgaaaaatgcaaaagaatttacaattgctcaaggtatcaggtatctagtgagcaacaaataaaaaagtaggtagaagctttgtcatacgattttgaaagtttggtaaaataattctcactaagatctctgtaattgcaatggtcttttttgaatttttagcaaaaactctagcgtcaatctcaaaatgttcattgttctgatagttctgatcacgcatcaacgtgccacccaagcggattaaatctttcttttgattcacttccttcttaagtcacgcatgtgatatattatcctcgcgtgatctcttggtgcacattatactttcaatggcctatacatccttctaactttttataatggaaaacacaaaataatattctttcttcaatattctggtttagaactgagttctcttcaatccacattttcaaccacgattttctagcaaaaatgatctacatagctcatttgatgtactaatgaggtccctcatgaattgtgttttcccatttttcacaatgtatatcccctcaccactttcgcacgcaaagttgtttaattgcaactcacttgaacttttcgaatcacaattcaggtgtgatgcatatcaatttatgaaacatatcttgagaaattgggggttgtaatgggacatgattattgctactaaacattccctgagcattcaaaacacaagattcttacttcaaaactactattttacaactcctgtcgacttttcatgttatattgcatctatattcacaaaaaaGGTCCAAAATCGGCAAGATCATTGTTAG


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Restriction site: MluI/SacII

Tissue specificity (embryo): Seam cell

Tissue specificity (L1): Pharynx, Rectum & anus

Number of insertion lines: 3

All strain names: SYS1517, SYS1518, SYS1519

Representative strain name: SYS1519

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage