microRNA name: miR-228

WormBase ID: WBGene00003321

WormBase description of miR-228: Expressed in germ line; neuronal sheath cell; and seam cell.

Conserved microRNA sequences of miR-228:

D.melanogaster: dme-miR-263a, dme-miR-263b

H.sapiens: hsa-miR-183, hsa-miR-200a, hsa-miR-514, hsa-miR-96

Location: Intergenic

Promoter length (bp): 2001

Promoter sequence: gagattcatattcgatttttccctttagacctttccgaagctggccgacaattgtcttgaaaggggtttccaagggataggtggccgagtttttgtattccgcgaacacatattaccgttttgggaacttgatagccggcgtcaatttaagaatacgcggaataaaacataaatcagatattatcctacgttaattcaaaatattttttgaaatttgtttaatgtttcagttcaaatttatgtatctttcgcaaacgtttctgtagttccttttttgctttcgtggaaggattcattttgattgtaaaaaacaaacaaagtttcatcggttagaactccaattaaaatatttttaccatttcccacattataggaacataagtattcttattttgaagttactttcttggagagtaattaacgggaaaccttatgcgtaaaattttcaaaaattagcatgtttcaattgataagactcaccggataacaaaactatcagtagaattaattaatatgaatgttttttaaaatatgcatgaaacagtttttttctcaattcaaaaattattctgacttaatcaagtcataatatttagcataaatattaagtacacctggaacagaagtaacattaatcgtagtttttttgacagaaatgtaccatcgaaaatgattacataaacatttattgtaacatttgagttagttaactggacctaatgaaactgaacattatggcagtgagtctaatgaattttggtctcaaactaccaaaagttttcttatattcaggaataccacaaaaaaaacattaaaaactccacatccacgagagttgtattctcaggtagtttcaaattaaactttttctttgatagttttggtcttaaattcatcggactcactgccattatagacattatttattcggtttttttaaagaatttattaaagaactacaatatatgtttcttttattgttggtggaagtttgcgaatttttagactagcagatcctcatctggtaatttttaaaatttacttccacaaactaatttattttgctgttctcacgtatatagaaaataatttaaaaattaaatttgaattcccgcgtaaaaccatttgcgtgcgaaattcaaattcagccactttcgtcgattttaatggcgaaattttcattggtgacggatgttcccgttttttaaattgttttcatagttgataagctaaggacttaataataagtattttcccttttgaggccttcttaattatgttctgaaacagtagaaataagagattccaccataaatcgtgttcaattcggataatccttgtgtgttcattttcatgacccttcatagtgagggaataattggtctctctctctctctctctctctctctctctctctctctctctctctttttctctaaaactctctcaatcccttacttttcaacgacctcgacagtatttcaaatgtacacagaacaatagaaatcgcctcgtttctgtttctctctctctctctctctctctctctctctctctcttacttcttccaattgaatcgacaattttcgaaatcgaaataggccacagtgaggaaggccgacgaattgagggaaatacacagaaaccccccgccccataatctgtagagaaaaaagagagaaatgggcggagcttgcttactcttttggcgcctccttctctttctctaagtctcactttccatctgttctttcttctccacatacagtcattcgttggcctgtgctgcctcgtcgactactactactgccttcaaaaattcttttattcgtcgcctcccaaaaactgtttcgcaacttttctatttttttttgttgctttggcgagacattttcctCCTTATCCCGTTCGCAATGGCACTGCATGAATTCACGGCTATGCATAACGACAGACCGCGGATCATACGGTACCATAGCGGACGGTGATGAGGTTAATgaagta


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Restriction site: ApaI/MluI

Tissue specificity (embryo): P cell

Tissue specificity (L1): Intestine

Number of insertion lines: 3

All strain names: SYS1516, SYS1583, SYS1584

Representative strain name: SYS1584

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage