microRNA name: miR-235

WormBase ID: WBGene00003328

WormBase description of miR-235: Expressed in AMsoL; AMsoR; and hypodermis.

Conserved microRNA sequences of miR-235:

D.melanogaster: dme-miR-310, dme-miR-311, dme-miR-312, dme-miR-313, dme-miR-92a, dme-miR-92b

H.sapiens: hsa-miR-25, hsa-miR-32, hsa-miR-363, hsa-miR-367, hsa-miR-885-5p, hsa-miR-92a, hsa-miR-92b

Location: Intergenic

Promoter length (bp): 2522

Promoter sequence: ttttttgcagcttcgtttcagcgtgaatcgctttgtaataaaacaacctgttagctggtcattttggtcacttaatcgtgttcactggttcattgagaagagaaataggagatgaaagggaaaacatcgcttcattcatcgttatgtcattgtgaaaggatacacaaaaatgggccggcccaaaaaaaagggtacttattgcgtttgacttccttaagaatagtcctattccaagtttgactactgatttaattttattctttctagtaaatttcttgtaattgtttgaagcgagtttagattttatgttatgaagatattccgttttataaacttttaaaagctaattttgtttttcgtaaacgtaggcgctcctagattagtttaaagatcagttattacgataaaaaagttcacatggcatttgtagtgaccacgtgacgtacgtaggtcgcctttaaggcaaccatgctattgtttcgtgcctaccttcaaataaaatgtgaataatactagttcagaacaattttagtcctgtgtgtttattttcttctattttgaagcacgatttatctgtgaatgttgaatttatgtaccacgtctgattatcgatgctcatttccatagaagtttaaaataaattagatacaggaatgcacattcttctgagttttcgttaatcgaatatctcgattaaaacctgcagcagtatctaatatttttaattgaacaagtatttctcatcatcctagaaattcttcaaatgttcatctttccaatagaacatacaaatcacacgacgaacgaacaattcttcgtcttcagtttttctcaaatcaaatggtgtgtatgtaaccaaaccagacacctttctccttttcttagacaccatacaaaatggagagtacccggagagaaacaaatgggcggaccttcaattggtaccaatcgaaactcgttttcttctaagtgtatgtatgtgtgtcctttcttcagatgacaccacctgtggatggttcttaattttctatctattccttattcttccaagtgctatctattcgtttgctgaaaatcgTCCGAAGATATCAGGATCAGGCCTTGGCTGATTGCAAAATTGTTCACCGTGAAAATTA


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1): Hypodermis, Seam cell, P cell, Rectum & anus

Number of insertion lines: 2

All strain names: SYS1751, SYS1752

Representative strain name: SYS1752

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage