microRNA name: miR-236

WormBase ID: WBGene00003329

WormBase description of miR-236: Expressed in pharynx.

Conserved microRNA sequences of miR-236:

D.melanogaster: dme-miR-8

H.sapiens: hsa-miR-141, hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-429

Location: Intergenic

Promoter length (bp): 2001

Promoter sequence: ctttaaattaaaacggtgagttcatattaagtttgttcattgaaaatgtgaaccgagtatttttcaacttcatagtaaaaacgttagaagtcaattccactttcatcatagggaattcagcctaaatgcactttttaaagtccatatatgacactacaataattgcagtaaatgttttggtaagttgccaaattttccagaaatgtaatttcgagcattgctgcatccccaggaattttttaaatacaaacaactaaaacgcaatacaatttataattgtttgattgtatttcaaaattataattggtgacaactgaaaacttaccactcttcgaatgaaattttttaaaaatgtaaaaggcattttagcaacacagacgcggcttttttaaataactttcccaactgacgatgcaccatctttaaagtagcgttatgttgttcagagtgaacctcaccacgatctcagttaaagtagttacaaaaaaaaactagactcacatttttagagaggaacgataaatatctgcaaaaagttactgttgtcgtgatggtacccattctagaagtgtttaagtccttacagatgaagcttaaattcttttgaaaacctcaaaaaatcaacaattcaattttcaaatgtttgtctgtgttctctccatctctctttcaaaggtatgattactcctcaacctgcaaatgcaaatgtgctgatttgttccgttatcaatcccttttccgtctgtctccatttttgcccgtggttacccttctctacattatacttaacaaacatttcgtcttatttcaaaccaatacctttctcctcatcgtcgtcttcttccttcatttgtgcatttagctatcaaaatgaagaaaataaaggggttttcactctctttctctctccatctttctcttcttctaataataattgccaggtgggtgcccacatttgtgtctctcatgcgaaatgatcaatgattacgtttgtctgtcatcagaagcgtaaggttttccgaacgacgacaaatgagagagatatcgagttctggtttctcccgtcatcacatatcatttttgttttttttttcattttaattataatgatcacaaattttcctgttttttttttgacaaattcaaacctactaaaacaaattcaaacttactttgaaggcgcatgcattttttattacccagttgagcaacttttcgattttgtcttatatcccggtctcggagcgaaatttcgcagctacactactcgcaagtagttctaaagtttgaacaaaaccaccaaaacctacggtagctaaaatgttctagtcgtgtcgagacccggttgtgtcaaaaattgaaactgggtaaaactcgtacgttttacccaaactttaaaaacatcacatgatcctttttcgaatttacttttagtattgttttacacgcattgcttagcttttttgtgaactatgaacaatattccctgcaagaccaaacacctctcaaaaatgtgtgtaccgggctgtcagtaagggcctgtcgctaattaaaaatgcctataatagttgttgcccgcgtgacaagacccatgtgcgtaagatctcgtgaaaattgccatctttagagctctaattaaatagagaaattcgctatatcataaagttcgagctttaatcaaaaaatctttaaagaatgctctgaaattcggagatcctaatataaaaaatattttgaataatttagtttccattctcaatccaacactaggtgcaaaacttccatctatcgggaatcgtaatttttaaaataaaaagcataaaatgctcaaacacttgttctatttgctcatctacaaaagcactgtaagtggtcgctccggttgctccgcccattctctcccactcactccgcccttcttattattgctctctatgagcacacgaggaccccatcggctcaaatcgtcattcttattcggctactgtcgtattctTCGGT


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Intestine

Tissue specificity (L1): Intestine

Number of insertion lines: 4

All strain names: SYS1443, SYS1444, SYS1461, SYS1462

Representative strain name: SYS1461

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage