microRNA name: miR-244

WormBase ID: WBGene00003338

WormBase description of miR-244: Is affected by several genes including eat-2glp-4; and prg-1 based on RNA-seq studies. Is affected by graphene oxide and nicotine based on RNA-seq studies.

Conserved microRNA sequences of miR-244:

D.melanogaster: dme-miR-9a, dme-miR-9b, dme-miR-9c

H.sapiens: hsa-miR-9

Location: Intergenic

Promoter length (bp): 1531

Promoter sequence: cccccctttgacaatcgaataattattttaaacaaataccaaaaaagcgattgcaataaaaaaaattaattcaaatcattttttacttttttacttgtttcaaagaattcagatattcagggaactgacaattttaggtttgcaaatttctcaaagaaatgttcttggcaaagtactggttaggaaggcaattttcgagataaaaatcttaagtggcgaaaacatgtgattttttggaaattggagatgagaaaacaactttttccgaaataaatttgaagttcagaagagaagttttcagaaggaaaacattgataatttttccagatttcagctttcaaaaagactagttcactgcaaacttgctcagatttcagggtttttacaaattattattattcgtttaagatatcgcggatttggtacacaacgacactctgatatctcaccgcgaattgactaatctacagtactcagacatcaaaggcgcaggtttttgtcttatttttcagaaatagaatgcaatgtcccccgtggatttaaaaattatgttttaaaaaaattttgaaaaatactgagcctttaaagagtactgtagtttcaactgtagtactgtaattgttgttcaaaccttttttgctgtggatttttttaaattttcacttttttgtgtattttttactagaaaaccgcataaattttaacaaattatgagaaaaacaatgaaaaatcaatggaaaatcagtagcaacaatatttttaaactacagtactctttaaaggcgtacactgcttgtattttacaagagaaaaggcgtgttgagacccggtaccgtattctgggcacaaaaccgcgaaattttgcgtatgcgtgatattactgtacttcttgaggacgcaaattattttttcatcagaaaatattcggtcgggtcgagtaactttaagagaaactgactttttcttaaaaacatatctgatttatcaattttcaaacatttccagttccaggttggcgttaacatttttcagaactcttaagctatgcttaaaccgggtgaaaactggttccgaaaagtgcgacctaattaaaaaggaaaagtttataaattttgcatgatatccgtggaaatctcgaaattcgggattctaatcttaaaatctttttcctccccgccaaaaatccggacatattccatcgaaattaaccggatctccttcttcttcttctcgttttcactcattttccttgtgtttccattttgcctcaaaaccttctatctcaaacggatctcttttcgatgtgtgtgcgccccttctttcctcagcggggtgcccattgtccatcttacagtgtctcgagagacacgcccactttccccgctcgcctgattttgatctcctcctgtgcggtataaaatgggtgatgagatgaagtagaaggcatcaattacactattcttcatcgttttcatttatttctaaatattccattattttctcacttttaattttaattttttccCTCCAT


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Neuron, Glia, Hypodermis, Pharynx, Muscle

Tissue specificity (L1): Seam cell, P cell

Number of insertion lines: 5

All strain names: SYS1453, SYS1495, SYS1496, SYS1497, SYS1498

Representative strain name: SYS1498

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage