microRNA name: miR-247 miR-797

WormBase ID: WBGene00003341 WBGene00045358

WormBase description of miR-247: Expressed in neurons and seam cell.

WormBase description of miR-797: Is affected by several genes including prg-1alg-1; and alg-2 based on RNA-seq studies. Is affected by multi-walled carbon nanotube based on RNA-seq studies.

Conserved microRNA sequences of miR-247:

D.melanogaster: dme-miR-279, dme-miR-286, dme-miR-996

H.sapiens: hsa-miR-134, hsa-miR-708*

Conserved microRNA sequences of miR-797:

D.melanogaster: dme-miR-11, dme-miR-13a, dme-miR-13b, dme-miR-2a, dme-miR-2b, dme-miR-2c, dme-miR-308, dme-miR-6

H.sapiens: hsa-miR-499-3p

Location: Intergenic-operon

Promoter length (bp): 1788

Promoter sequence: aatttggaagaagaaaaatcaatcaaatttgaatatgaaaaagcgtacaatgagaggccacagatgttttgattgcgagacagaagtctcgtattgcaatcggcaaatggttcacacatacgaaaaggagaaaacgacaaaaaaaaagaacaacacaaaaatttatgagcaaaggtgcacgcgctcacgaggggggccattgatttaacgcgtacattttattgatatagggaaaatatgatgaataaccacagaacgcgtactagagagtctgtaacagggaattatagggcaaatgctcatttttttgaaagctgagtaagtcggcggtcaaaaatgttggccattgctggacttataccgttttacagttgtcaatttcaagaatcgccgcagagcaatcaccacgcaccttttagtgggtataatttttgaacaagatttatctcgcaatacacggtaagccatgattatggtagcacgcttccagcagtacagtggtcgtggtaaaaataattcggacaactaccattttttattgagcacattaaccgtttaaattttgattttttcattttcagaactttcggtgggcttccttggcgccctcgaggacacgttaattttcatagagtattttccgtattgaaaatttctagatatacttttttggtacttctatagttgccgcacccctaaaacttaaaatttttaaaaatttctgtgaccgttatttaacaatttcaggcttagaaatgatgcttggtgggtttaagtactatttcaaccggaaatatatagatagttcatgtttggggtatgactactacataaaaaccattttgaaatcagtagagtttggttataaataatatgagtttttaaactatttacaaatacggtattaatgcaaagaaaacgtcaatactcaaaatcgtcaaaaagaagtgaagttcgacctcgacataattcgtctttttttataaacaaaaaactcaatatttcaaggttcaagtcactttaaggttaaaagtgaggattttgtcacaaaatacttgtgtactatgaaaaactataaaactttggatcctttcaagtttcagcaactgctggtattagaaattttcggcaatcggcagttttggcaattgctgatttatttaaaatattcggcaattggcagttttggcaattgctgatttatttaaaattttcggcaatcggcaagtttggaacttggtgaaattgccgacatttcaattgccgaacagcaattaccacgtgcctgatttttggaagtttcgtaaactatatgttggcatagcggggctttcaacagattgatcatttccatatttgaatttagaaaaacgttacgatttttttgacgtattttaatatacttcggctatcaatttgccaaaaattgttttgacaatagtcgattcctttccatactcaaaatgtttataacaacattaaatttgttcagctttatttctaatatcacccaatttttttcaactaagctgtctgtttcaacccgtccttcaacagctgcattttttgtccagttacaagatgttttcttttccactaccagtgttaccaccgcttgctacaaacggcaaaaaatttgaaaactgactagaacgcgaattggtaccaacacataaggtcagcaaacaaaaaccctcgccactacgccatgtccctttcgtaactccgcccatgtcgcatataaaagacggttcaaactgaacactagcacactagttgtctacaaAT


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1): Pharynx, Rectum & anus

Number of insertion lines: 5

All strain names: SYS1457, SYS1463, SYS1464, SYS1465, SYS1476

Representative strain name: SYS1463

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage