microRNA name: miR-252

WormBase ID: WBGene00003346

WormBase description of miR-252: Is affected by several genes including eat-2alg-1; and alg-2 based on RNA-seq studies. Is affected by Microcystin-LR based on microarray studies.

Conserved microRNA sequences of miR-252:

D.melanogaster: dme-miR-1002, dme-miR-252

H.sapiens: hsa-miR-26a, hsa-miR-26b

Location: intron anti-sense

Promoter length (bp): 2072

Promoter sequence: AGATGATATTCAAGACATTATCGACACCCACTGCTtatcacctttatagtctttatcaggacattttgatttgcgtccggacatgctctcgcaatccaatttgtatgtacattcatttaggggtttgaacaggaaaagtgggtggcgaaacatataaattattagaaattagaaagttctagcctccagttggtgcttgtgctccgagaattccgattccactgttgacaccgacgagtgattttggtggagcgtactttggaagagggatgaagccgagctgtaatctacagttttattttgaagtattgtaatttaaagggttttcggtagaaattttggttttgaaaaaatatatagtaaaaggaaaaaactgtaaaaaatgaaggtcggaaaatttcaaagtttttgtaaattaccaaatttttcaaactttccaaaaaagtttccagaatatttgactactctaaaatatcctttaaacgtcaaaaatttacaacgatttagtctaaatacacctaatatactccagaaatggtaaaattgtataatagaaatccaaaaattttcagattttataaacttgcaggcaagttttggtaaattgtcaaattttccaaaaattaagactattccgcaaaaaaactgcttatttcaatgtgataattctactttttgaatccaaaatttatatacatagaaatgcaataaaacattggaccgatctgagaataaaacttattaatgtcaaacagtttcaaacagggtctcaaatagtgtctgaaagttgcttgttgccgaaaaagtgttgtgaaccagcgactccggcactatttgaaagctttttttttgaaactttcatcaagaatacattttattctcggattattcaaacattttactgaatttctatattaatttgcaatttaaaaagtaagattttcgtatagtccttatttttgaaaaatgaacacttggaaattttgccaatttaccaaaactatgccagaaagtttataaaatctaaaatttattggatatatttttgagatatttccaaatttcggactatatgaagtgtgtttagactaaatccctactgtcgatacctgtaaatacagttcaatacaatttagatacatatgagcactttgaactagaaaaaaggtagatcagaaatttttagacccaaacgtagatttttgacgttttcagggcatttaaggatagacaaatattttgaaagtcttttggaaagttttttccaagtattgaagtgtgtgaaaaatcttctcaaaatgaatttgcataaaccaaacagccttacattaaccgcctgtatatacttcatcgcttttccagacacaatcaaatccttattcccatcgtagtcgttcaatgattgttgttctccctggaaaaagaaaacaatacgaatattcgtctcctctgtcttttctcccgttagcctttttttccgttcgtcttcatattctcgatagaaacaatcaaaaaaagaacgaagcgcgccgccggcaccgtacgaagcgaaaacataaacatttgatagtttgggaatggggtatgcaaatcgcatacaatttcttcgtttttcttatttcttgcagttctctctgatagcgcccgtcaacgtggaactaagaaatcgagaaccgccccactccacaccggctctttcaattcacttctatgctcacttttattccataaagtgtatgtatggacctactaatattactactctgattgtacataccaaacacacagcggggattcttttattattataaaatcatatgcatatacaggcccctactaataatagtcgtggcacccccctacatcccggaaatagtatggtgaatagagagacgccgagaaaacgagagagccactgtagcaatctttgtgtgcacagcttccagcacgatatttctttttttctctcggctctctagcactgttataaaaggattgaatgggaaggcaaaagtcgtcattctgatcttactatTCCAGGGGTCTCTTTCAGTTATAAGTAGTAGTGCCGC


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1): Intestine, Excretory system

Number of insertion lines: 3

All strain names: SYS1427, SYS1456, SYS1491

Representative strain name: SYS1427

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage