microRNA name: miR-2

WormBase ID: WBGene00003261

WormBase description of miR-2: Predicted to enable mRNA binding activity involved in posttranscriptional gene silencing. Predicted to be involved in post-transcriptional gene silencing by RNA. Predicted to be part of RISC complex. Expressed in neurons.

Conserved microRNA sequences of miR-2:

D.melanogaster: dme-miR-11, dme-miR-13a, dme-miR-13b, dme-miR-2a, dme-miR-2b, dme-miR-2c, dme-miR-308, dme-miR-6

H.sapiens: hsa-miR-499-3p

Location: intron sense

Promoter length (bp): 2013

Promoter sequence: TGTTGAATGGGAaagtgccgccagatggtgtgatgatgatggtaacaagggcgtttgtgaaggagaagagagatggaaggcaacacatggcgccgaagacaaaggaccgggcagctgctgctgctactgcagggctgtcctcatcttcgttctaccctcctgtcgtcattgcgatcggtttcctgacgagattcgttgattcaaaacgtgattgaagatagttagagagagagagaaagagctcgagtaatgctcggatcgattgactgaggatagttgtgttgtatttgagtagaggaaaagtgataaggatgcgcgaaggccggaacacgcacttgagtgccgcctatcggtaggcaacaagccggttattttgtgcacaaaggtagaaggtagaagtgatcagccagtagggtattcatacacggagataatagttaggaaagttgtgacggtttcgtagatcaaaaatgcactgtacaattgttcttttaaagttcaaccacagtcatacttctttttgaaaaacaaatttttaccgattgttacatctcattcaaattatacaatcaattttttttagtgccccagtcatccagcaatcggtaacagagcacactaggttttatggacctttttctagtcacttcgtagcggcctattttattgcactaccgcccatacgaatacaaattgcgcattcgagtattatgggacccttcattccatccgccatcgtgttgtgttcgcaccaaataacagacggaattgagaacagcagacgggcggccgttttatagatgctccgtcgccagatgtgttctcttggattcattagccagctcggttgaaaaggaattggaggaaacggcgttaatggtctgatagatttatcgaaaggattcttggagatgtgtcgtttgaacaataatcaaagttattggctaatgaaataacacagcagagtcgtattagaaaagattagggtagtgtttgaagtactgtagtccgcgttttaaaactgtattattgaaacattctgatctagaaatgttgaggtttctgaagtgacaataccccattccaaatagtggccatttcttcaaattgagctaatacaatgccaacatttccctcacagtataaacttcctggaaacatttgctcttctccattgcttctgctgccgttgtcctttcccttcgatattcactttaactcggaatatttcatgcatatttgaggggaattgcgtgtccgctgcttttcgtttttgaatgaagaaggtccctttagggtccgtacgtccgaatagcatgagcggagcattagaaggaaaaagagagacccagatgatggaattgcgtgtgtgtgtgtatgcatgggctcttcctttccaagattgtgccgcttcttcttctacttctcgatgagtttttgtctcctagttgtcgtccttctcttctagtcgtcttcgttttttcttcctttttttccagaaagtagttcgcgtcttccctccgccttggattaggatcaccctgcgatgtgtatgtgtctgtaccctcttcatagagcatactctggaagtgggcgggaatccggagcatcgagatagtaccaattgcttgaagagattcgaaagatattgagaagtaggaggaggcaacactatgcggagactccgcccccaccacaaccgccgcccggggtgtttgtgtggagtgcgcgtgtttgtgtgcgagtgtgtctactgaagagaaggctgctgccgtcttcttattcttcttcttctcgtcctctactgctgcagcagcttccgagggtcccgtcgtccaacttgtattgagccattggtccgctctatgtgctgtgtgtgtgtgcggagacatgaaaagcagtcacatgcccccagagcggagctcctcgagccctcacttcttcttcttcttcgtcttattctacctcttcgtattttattcttcgctaccacaatcgtatttcgacgaatcT


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Muscle

Tissue specificity (L1): Neuron, Muscle

Number of insertion lines: 2

All strain names: SYS1559, SYS1561

Representative strain name: SYS1559

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage