microRNA name: miR-43 miR-44

WormBase ID: WBGene00003271 WBGene00003272

WormBase description of miR-43: Expressed in germ line and seam cell.

WormBase description of miR-44: Expressed in seam cell.

Conserved microRNA sequences of miR-43:

D.melanogaster: dme-miR-11, dme-miR-13a, dme-miR-13b, dme-miR-2a, dme-miR-2b, dme-miR-2c, dme-miR-308, dme-miR-6

H.sapiens: hsa-miR-128, hsa-miR-27a, hsa-miR-27b, hsa-miR-499-3p, hsa-miR-768-3p

Conserved microRNA sequences of miR-44:

D.melanogaster: dme-miR-279, dme-miR-286, dme-miR-996

H.sapiens: hsa-miR-134, hsa-miR-708*

Location: Intergenic-operon

Promoter length (bp): 2006

Promoter sequence: CATGATtaattatggatgtgcgtagagttattaccgtactattccaccacaaccatctgccaacattccgttgcgcgcatcttgatgacgtggatgggtggcgatggagcttaattatagggcacaacaatggcggacatttttatgtgacaagatactgtcgttttttgttcgtatgaaaatggagattaagatggaaggaaacaggtggtgaggcagatgtttgaagcaaaattgacaggactcaatgagcagtggtggggcgatctgaaaaatgtgagtattaaggcattgcgtgcctgcctacgtgcctaccaggtaggcttgtttggtcaagagcctagagtaggcagtcttctagactagcctggcaacctatgggtccacaactctggaagaatacctaaaaaggcccgtaggcaggcgaacctgacacatttctggattaacacttatcttctcaaataatcttgagacatcttgcgcaaagacgctgaggtactaggtacgaggtactcaccaccttccatagatcctcagtagataagaaacttttgacgatcttgatttgtgcagcggaatcgggtgattcacctcgggaaatgaacatggttgggagaactgagaaagttgcgaagatcacaggaaatttttatcggacttcgttacgccactatatacattcgatagagaatagttacagatgatcactccaaaaaatttagctgcttcagagcaggttcgaccaagttacgacattttgaaagtgccaaaatactcctttttaaaaattccaaaaaattcaatttttttttattctaaaacttgatataaacgttttcaaatggttggaaatgatggaaaaatcataccatacaagtttcagatcataattctcaaaaaaaaaacccttgaattgatctacatgaacatctattttttggaatttttctcaagatgtgtagattattttaagggtttttttgagagtgacgggctaaaacttgtataatgttttatatgttttttactgttttaaatcgattacaactatttttatcaactttcagaaaaaaaaattcttagtgaaaattttagatttttttcaaagggggtgcatgaaggattttttgtcggcaactttaaagtgtcgtaacttggtcgaacctgctctgaagcagctaaattttttggagtgatcatctgtaaccattctctatcgaatgtatacagtggcgtaacgaagtccagagccttcccttgttaggtttagacttaggcttgggcttaggcttaagcttacatttggcttacattagaattaaactttggttttattcaatttttaacctgaatgattggtggaaatttgaaaaaactgcaaaaagttaaatttggaaatttccagtttttctctgaagtgatctgctgctctgatctcttaaatgtagcccgtccaaacatttttgagggtgctaatttttagttttgcgttcagaatatataattttcaaatttaagttcttccaggagcccgtttctatgggctgaaattgaaattaggagcctggagcttgcacttccaaaacccgacgatctgagaaatccatgcaaaacgtcgtctcctgccttttgcatgtccttgactacatccatctattctcctcttcgacgcgacaaccaacacaacggtcgaatcgaaaaaagcgaccgggtgccggccatctgccctccttccctttccctctatcttccaccgtctgcatcctcttcacctcccacactctcccgccccaacccatgaatcgggtcacgcacacagtaccggtgatggtcaccgtgtgtatatgtcctcgatgtaaataccgccccttttctccttctctacctcattccttattcagactatcttgtgcatgagctcagggctcttctgggctcgcgatgtattccttattccttattccttcaaacttctgaaattcctctaaaatccccataaaccc


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Neuron, Glia, Seam cell, P cell

Tissue specificity (L1): Neuron, Seam cell, Rectum & anus

Number of insertion lines: 3

All strain names: SYS1365, SYS1534, SYS1522

Representative strain name: SYS1365

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage