microRNA name: miR-45

WormBase ID: WBGene00003273

WormBase description of miR-45: Is affected by several genes including eat-2; prg-1; and alg-1 based on RNA-seq studies. Is affected by multi-walled carbon nanotube based on RNA-seq studies.

Conserved microRNA sequences of miR-45:

D.melanogaster: dme-miR-279, dme-miR-286, dme-miR-996

H.sapiens: hsa-miR-134, hsa-miR-708*

Location: Intergenic

Promoter length (bp): 1561

Promoter sequence: gccgattgtgatcaaaaaaatcaacatcgcaactgccacgtggctttgtttttacgtggttgcgtgttttatttttagatacaggtttttaaggaacacgccgataaataatagcaacggtaaaatttttcaaaaaatccagaaatacacgtggcgtcagagtgtcccatgccggtttgatccaggggtgggcggcaaacgatttttccggcaaatcggcaaatcggcaaattgccggaattgaaatttccggcaaatcggcaaaccggctaattggcggaattaaaaatttcccgcaaaccggcaaatcgctggaattgaaaatttccggcaaaccggcaaatcggcaaatcggcaaattgccggaattaaaaatttccggcaaaccggcaaattgccgatttgccgaaaattttcggcaaatacggttttgcacttatttttcgaaacttcagaattccaatttcaaatgacaaaactgaacacatcctatgaattctcttacatctattttgaaaagcaagcaaattctatgaaaatatgtagaaaaaacgggaaaaaattcaaaaaagcacagttttcagtgtttctgtcttataaaaagtccctctaaacatttccggcaaattagatatccggcaaacggcaaatcggcaaattgccgaaaataaaaatttccggcaaatcgacaatttgccggaatggaaaatttctggcaaatcggcaaattgctggaatttaaatttccggcaaacggcaaaccggcaatttgccgaaaataaaaatttccggcaaatcgacaatttgccggaatggaaaatttccggcaaatcggcaaattgctggaatttaaatttccggctaatcggcaaattgctggaatttaaatttccggaaaatcgacaaaccgacaatttgccggaattaaaaatttccggcaaatcggcaaaccggcaatttgtcgatttgccgaatttgtcgacatatcaatttgccgaacgccattcgccgcccacccctggtttgatctacgtttgagaagtttcgtgagcgccttctctactgtatagctaatttctgaaaagtaatggaaacgagaatttgtagttttttttttgaaaactgactttttccgcgaattcaaagttagttgatatttttatattgtaaaaaccttggtgggtctcgccacgatttttttgtacatttctaaaatataattcccatttcccaggtcttgccacgatcacggaatacctttttaaaaaaggtgtttttttcagattttccagaatcttccagcttctcgagacacgatgccccgcccctctcaactttcgactattttcaccttcaccccaaactctccaactttttccttatcctttcccctgttaccaaaaccccgcccagtttctccttcttctctcaaacaggtcggtctctctctctctcttgacattcatttccttttgaccctttcactcgataccgccatcggccaaacttctgagaatcaacttattcaaatttcgttccagtattcagctgctcatgCACC

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Tissue specificity (embryo): Hypodermis, Seam cell, P cell, Intestine

Tissue specificity (L1): Hypodermis, Seam cell, P cell, Pharynx, Intestine

Number of insertion lines: 2

All strain names: SYS1468, SYS1469

Representative strain name: SYS1468

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view