microRNA name: miR-49

WormBase ID: WBGene00003277

WormBase description of miR-49: Is affected by several genes including eat-2; prg-1; and alg-1 based on RNA-seq studies. Is affected by nicotine based on quantitative PCR studies.

Conserved microRNA sequences of miR-49:

D.melanogaster: dme-miR-285, dme-miR-995, dme-miR-998

H.sapiens: hsa-miR-21*, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, hsa-miR-593*

Location: Intergenic

Promoter length (bp): 2000

Promoter sequence: tgcgttgtattgttatcatcaaaaatacaaacccaaacacacacctatacattgttcttgtgcccattacaaacaggtgaagagaagaaggatcgattatgtggactgggtaaacatagaccgatggacggcacccaaaagatgtgaaccactgataataaaacaagcatgcgtgaacggcgaaggtaacgtgtttgaaaacgaacactttgagtgtttaaaatgaaaacattaaaacacttttaacaactggagttttgaaaatacatattttcaaaattcaatcagcctcctgcaaagacattgcatttggggtgagactacatctaaatatctgaaatcaaaataaaaacaatgatttggccagttttgtttcagtaactaaaaagaatagcatgatctgacaatcaaaaaactgtcttgatttgaagtattttaaaaccactttttgttttgcaaatatgatgacgcagcatacgtcaagcaatttggaacttttggtaaactttaaaaatcaccgttcaagttcacagaattactaaaaacaaaatataggctttgcatctaacctaactgaataacctaactaacctaactgaatgaatggctttactgctgttccactgttttgattgttgtttaaattgggaaaacgttgaaattggcatgcttaaaacacgcaagttacaataagtctttacattactttcactttaaattcaattttcaactacttgaatttggtcgttattgaaatttcattcgtaaaaaaaaatactacttgtatccgtttgtcttcaatccattagcaatttcttctgtcttttcttttaccatcgtcatttaaattattgcatgtataattataagtaaggttggatgagacgatgggaaataatatccgtacaattatccatatatggtgtctctcattttttgggtggagcggcatgaacagtgaggcacaaaagcacacaatacctgcggcaggtgttcaaactgaaatttgaatggcgatgatgacccttcggtattgacatactccaagttcattgatttttagaaaatgaaaacgtcggagtgaaaacacattcaatgggaaaataaaaattggagcgcaaaaaagcccatcttgaaagcaagatggagaaattggtagggtcgtaaattttgagaatttccatctaaccttctcctccatgccaaatactaaccaatatagtgcgttttcgcatatatatatatatatatatatatatatatatatactgaacatttaatatgttgagtatctattgaatatttatttatttgtaattgacaagccaacagttttcagaaatcgaacagacaattaatttgaaaatagaaaaggtgcttgagtttttgaagaaaatatcaaatttgaagctttgaaattacgaaccagaggaacattttagggtacataaacctaaaaccctaagattgtaaaaattaatataattaattctgcaaaattgcatttgttgtatcctgaacatcatcgaacatgtggacacaccttcgagaacttcatcccgttttgtctgtcttcgattaaagattgcaatctttttccctctccacctactactacttgtcgaacacattattctgaccctgcttatttgttctgaactcttgatcgataaggaattctcagtctgtttgtgcattgataaaaggaaaggtccaggaacacctggtaaacatttccttcctcgtcatcttgcctttcgacgatgcgaactagccacgacgtgtcataaataagctccgcctccatctcttcgtttcgggaggcggccgatgcaaggccaggcaccaccacttaccattcatagcgtgtatgtgagcgcgtcctcttcctcggcttgcctaccctgccatcgccatttccttgtgcacgtgttgtactgcacactctggtgttggaaaaaaaaaatgctggaaagcagtttattcagatgctagtgtac

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Tissue specificity (embryo): Intestine

Tissue specificity (L1): Hypodermis, Seam cell, Intestine

Number of insertion lines: 9

All strain names: SYS1564, SYS1565, SYS1566, SYS1567, SYS1569, SYS1570, SYS1572, SYS1574, SYS1575

Representative strain name: SYS1567

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view