microRNA name: miR-52

WormBase ID: WBGene00003280

WormBase description of miR-52: Expressed in several structures, including arcade cell; coelomocyte; gonad; hypodermis; and vulva.

Conserved microRNA sequences of miR-52:

D.melanogaster: dme-miR-100

H.sapiens: hsa-miR-100, hsa-miR-99a, hsa-miR-99b

Location: Intergenic

Promoter length (bp): 2020

Promoter sequence: GTGGCCCACATACAaactgcttaattaaaatttgcaatgatttttcaagaaatccagaaacagtgattgttatataataacacccagttgattttattaagttcataactaagtaaattgataacttccatttttggtgcagttccagaaaaggtatattcaattggtattcagtttttaatcttctcactagagaaatttaaatattcatcaaagagcattgtatacgcaatcgaaagaaaatattaagttagagcgaatcaaccttgtttcctgtaagatccgtaacagctcttgctactgaagcttttgagatttaaaaaacttctggccaaaaaaattaaattttaaaaaaccttatcagacattttggactaaaactgatagaaagaaagaaagcagatccctgacttagcaattgcaataatttataaatcttgaataaaaaatcttgaaacggtaattttttttccaaatttacactttctttttaactcattagcttcaactgttcacccagtggcgcatatacagactgctcccttaacctccgtcctagtcgtaaagtacatgtccattaagaatttcaaatgccaaattccaatttcccagcagcagctgcaacagccgttcgcttctcatttccgttcttcgccattttagaaatccccatttcatttcaaattcaaatgaccgccggccgattgatgaatgaatgaatagaaattggccaccccaaccatcacccagcgccctcctccttccaaacaaagatgcatttaattccatgggcccccggtctctctgtgaaatacacattcatctttcccccgtcctcctacttctcatcgtaaacgtcgggcggcgtatgtgcccttgaatttcatttgaattccgtatagacaacggaagagggtgaacagagagagacaagtgtctgcgtctcaagggtcaacacacacactctctctctctctttctcgcgagtgtctaagacgttgcgtttggatgcggttggcttgcggcacagacgtctatgtctgttcaggtcaacaaggagggatgaaggactatctctagacatctgaagggtctctagtagtgtctagttgtctgcgtctctcatcagcttctccctatcggtcccccaagtgatggcggcaatggtgactctctctctctccagagaatcttggacaatctcccccagagggggtcttgtgactctttccttgtatgggcagacactggtcaacggcatcgctctgtgagtctctgcctcttagtctccacacactccctcgccatctttatcgagaaggttcgacgtttctcctggatgtgtgtctcgggtctctcgggtcaccatggtctctagtgaatacttttgctgtctggaactctcttcgtgttccaatgatcagatggtgtctctccccctctctccccattccacatttcctcgatgcaacgcgcaccaccacaccacacgcggggagggtaaaagtacaacgtatagtggccgttctctgcgtctcttcagccgacaccaatctctccgccccgtctcttcccgactagtacgagagccgtcctccaaacccccaaccgctcttcttggcattacgaggacgtaattgctatcttgactctcgccgtctcgccgtctcttcagtcctccacactatctcgtcacgtccgcgcgttgtttatcgcgactgaccgctctctgcccgtcgcgcatctcatctccacggtgcctggtagagacatgccccgccccacgcgtccacctgcagtgctctgaccacgcccactcggtgacggggtcggtataaaagagacggggagggggcgaatcggcatatacacagcattcgtctccctccacccgtggccccatttctctcgttctccttttcctctcttttcttccttaccccccaaaccctgccgctactactactactcctacaactacaactactactactactatacatattcatgttctTCCAAC


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Restriction site: AflII/SacII

Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 6

All strain names: SYS1377, SYS1379, SYS1378, SYS1380, SYS1381, SYS1382

Representative strain name: SYS1379

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage