microRNA name: miR-53

WormBase ID: WBGene00003281

WormBase description of miR-53: Expressed in arcade cell; distal tip cell; hypodermis; muscle cell; and neurons.

Conserved microRNA sequences of miR-53:

D.melanogaster: dme-miR-100

H.sapiens: hsa-miR-100, hsa-miR-99a, hsa-miR-99b

Location: Intergenic

Promoter length (bp): 846

Promoter sequence: cggatccttaagtcagaaattgttgaagacgcctaatttttccagtatttcaaataactatagatctcttgttttttgcaactttcctagtgtactgtcatgtaactagtaatgtcactaagacatacatactctgttcaactccaattataattattgaaaaacaacgatttttcgtgagaaacatgattttctttttttattctcttccgtttccaatattcattttcataaaaatgttttctccggcccctttgcaactgcaatatacattgcccttgaatctcacaatctgggcagcccgtcgtagatctatgtctactttactctcgtgttgttggttttgggtgaatgattttggattgcaatagaaatcactaaaacgtcgttttggtcggcgacttttgaggtcaccatgaagaaatgggattattgctttcacaagttgtccctccacaacttaacatctaggattattaggattgttctagatatattgagatttgattctaattatgccagtcaaatcttattgatgatagtccctcggttcagttccatactccatatttaacattcatgatccctactgcccctcaataggatcaacacatctacgtctcttcagttagagccagtctctcattgacggtcaacggcattctctgcgtatctttgtctacattgctctctcgttccgttcgtcgagaatgttcctacggctgtctcggtttctcatcgtcctcttggtttgcagttgtggacaactcgacatcattcgtctcgttactctacattattcctatctctctctctctctctattattataaatttctcCCGATTC

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Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 3

All strain names: SYS1431, SYS1442, SYS1445

Representative strain name: SYS1445

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view