microRNA name: miR-54 miR-55 miR-56

WormBase ID: WBGene00003282 WBGene00003283 WBGene00003284

WormBase description of miR-54: Expressed in several structures, including excretory system; hypodermis; neurons; and seam cell.

WormBase description of miR-55: Expressed in several structures, including excretory system; hypodermis; neurons; and seam cell.

WormBase description of miR-56: Predicted to be involved in RNA destabilization. Expressed in several structures, including excretory system; hypodermis; neurons; and seam cell.

Conserved microRNA sequences of miR-54:

D.melanogaster: dme-miR-100

H.sapiens: hsa-miR-100, hsa-miR-99a, hsa-miR-99b

Conserved microRNA sequences of miR-55:

D.melanogaster: dme-miR-100

H.sapiens: hsa-miR-100, hsa-miR-99a, hsa-miR-99b

Conserved microRNA sequences of miR-56:

D.melanogaster: dme-miR-100

H.sapiens: hsa-miR-100, hsa-miR-99a, hsa-miR-99b

Location: intron anti-sense-operon

Promoter length (bp): 2001

Promoter sequence: tgataccaaaacaattgagtgttcaaaaaacctctccgacagataattcgatacaaatcaggattaaattaaaaaaaaagagacatgtacagtcaaaaaatttttttcttcaaatacgtggttttcaacatttaaaaaaatttaaatatgaatgacaaaattaaaataagaaccatttcctacgcgtaataccggttgttgagaaattcaactttttgtagctctatcataatttctaattctggtaatcctccatgataatataacttctgatcagtttattaaaaacggacttactaacaaaatcttcatctaaagcctttcaaaatcaatttgagttgcctttatctgaaaagttactcaagttgaagttatgcaagtgcaggtcttagttctctaggttgtatgtaacctaattttattgtttattttaatgtattttccaattctagtgcttggaaagcactcctcaatgctctgaaattttacttcttagctttaaatttgaatttgtagcaattgttgtgttttttagcagttaagttatgttagataagagtattttgcttgcaaatgctttccctttctacaatattctgttcgaaaaggtcgaattccgtctgtagaaagggaaaatggaacataaccgttcgatgtctgttccaatcttttcgaaataagagataacacatgaacctgattcctgttgttctcttggtttcaggtttcaattcaactattttgcgatggacattgacattaaaatccgaatttcttatgcaaggcatcgtcttatttcattttgatgaaaatgcggaacacctgctccgttcttgtccgggcaccgaacgggctacaattaaacgtaatccgactgggttgatggaacataattggaggtttgcagcggctcagacagatggtggtgccagccggtggataagttcaaatgtaggattagtatctatacaatgatcagaaaagagagaggtatgaggaactaaagtatgttgctagtggcaaccaaaaatagtattgaatagcttagataaatggttctaatattctcatactagcttatttatcatgtttgtcacattgtgattaatttgataaaaaatacaatatttgtgacaaatattataaaattttatggtgggcttgctgagtttcaatgggcgaaaactgttatcttattaaaaatccaaagataattgtagatcacaacttcactttttcgcttctgaaaatgccaaaactgccaaaactgagccttaaatcagcaattttattgaatcgttttttttcttcaacagagtactcctaattttattgtcactaaacatgaatttgttataaaactcgtacagtttttttttaattggaataatatctatatcggaatcatgaagaacattctttcagttttgattatatgatccattcatcatctcataccaatttgtccacttccgagaaattattctcccatatcctaaatcgcttctgcattcttctttagttcctgtccgttctcggcgcgtcgagtactctttcttaactttcctgtaatcggacactctcgattcaatgcactatttcaaaacgctcaccacaaggcagtgagcgtcaatgggcgtgcgttttcaatgtggtgagtgctgaagacgaaaggggcctcgaggaaaaatctccgcccctaaacgtacaataagacgggccgcttcacgtgatgcagagatcgatgcccgacgagagtgtgtgagagggtgcggagtgtgtcagacgacgaaacgtccgatgtggtatgtgtctctccacacgggtcgcgtggctgctcattcggtctcgcaagcgcaccaccgagcggtcgtcttctccgcttcgtcctatgcccccttgccgccccgtcccgtcctgttcctcttccaaattttctatcacttctctctacccctcttgttctgatattttcttattaatctctggaattggtaatGT


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Neuron, Muscle

Tissue specificity (L1):

Number of insertion lines: 7

All strain names: SYS1486, SYS1508, SYS1510, SYS1511, SYS1512, SYS1513, SYS1514

Representative strain name: SYS1510

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage