microRNA name: miR-57

WormBase ID: WBGene00003285

WormBase description of miR-57: Expressed in several structures, including P11; P12; body wall musculature; hypodermis; and tail spike.

Conserved microRNA sequences of miR-57:

D.melanogaster: dme-miR-10

H.sapiens: hsa-miR-100, hsa-miR-10a, hsa-miR-10b, hsa-miR-146b-3p, hsa-miR-99a

Location: Intergenic

Promoter length (bp): 2201

Promoter sequence: ACTCATAATTCAAACGGAACGATCGTAGTCTCCACTTGCATTCGTCCCGTCTCACCCAGAAAACGAATAAATTTTATCTTTTATCGAATGCGCGGTCCGACGAATAATGGACGGGCGGCGGTGTCCCTTTGAACTCATACTCAGCCATTTGAATTATTACGGGAAAAAAGGGGAGAAGGAAGACATTTTTTGTAAATTTTTgtctgcacatctacaaagaaatattgaaatttgtgagttatattccttcgaatatctttaaacgcgatttcaatctctttctcaatttcttataatcggtggtggctcataaatcggtgcagaaggtcgatggtgtcaacggaagaaagagaacaactgcgtctcgtacattttatatatctcagtgagaaaagagaacatttgaaaaaagtttcgccccgaggacaagcacatttcgtttcattcgattcgctttgtttcgaaagttttatgtctcattgtatagcctaggattactgtagttattgtggatacggagtttaagaaagtttacagtgttttactagttaaaatagttaaatattcgttactacccagacgttaaattttgcgatatttgcgccaaaagtaatgaacccgatcccgacacgacaattatttgttaaatgcaaacgggtgtgcgtctttaaagggtattgtaatctcaatctttcgaagctgcgaaatttccatcgatttttgatagtttttctcacgatttaatgaaatttatgtattttaattgataaaaatcacagtgcttttcgaagaaattgcagtacttttcaacggcgcacccagttttggttttaacgaaaattgtcgtgtcgagaccgggtaccgtattttcggcgcaaagctttgtgcctgggtaacatctataacatcacaacaatacttttttttcgggtaccccacaactttctagcattagccatcaaacatataaagttttgataagcaacaaatagtgataatatcaatatttctttccctattcattttcttcttttcaatgtttccatcacctctctggagtcattctacacatgttttatgtgtatgtgtacacacaaaagaatccatttttccctcattctttccgtctctcggagcaatacttctctctgtgtcaccttcattttatgagtttgcagacattcacaccttttcccattcaatcaagaaatgggcggagctttaggagacgtgatttcttggctccgcccacttactgcgagaaagctgtttaagtaaatgtgcagggagtagtctagaacttctcaaccttcttctcgagctatgataattccagagaaaatttcaactagcaaaaataaaattttccaaagttggagccccttcttgataattaagtttttgaaaaatttggaattttcgaaaaatttgaagccaggctcaaatctctacagtattatattataaaaattgtagaaacatttttttaaccgagctccaaaattatgacaggcgaaagctaagtaattgccacttttcaactgtaactaaagttttaaatattttttcagaatatttcgttatgatttttgactattctagacataatagggccatctggacagcgggaagagggaacttttattattagatatttggaaataatctcccttcaaccaacatcaattttctcgttgttactactgtaatttcaaatttgagcaagtgtggctgttccttggcattaaaacaatgtaaatccttaatacatatcacctgccaacactgcattttcgttttagaaaatgtcgtgttgaaaaatcacacgagttttttcaaatttatatctaccgtaacctggctcttctgtcaattctctctctctctctctttgtgatttaatcccctaacacctttcaacatacataacaaaagtctttcgtattctctttctactaccgccgtcactcaaccctccagtgtccttcgttttacgagttttccaatgaaaatcccctcgtcccgccgtccagaaggtcgttaaatttacgaccgtccctttctcccacaaccccccttcgtccgtccgccctcccggacatttgatgttaccctctattctgaacttcttcttttattctgggaatattctgttcatgatgatattcaaaaaggtatgtgaactcatcgacatgctcgtc


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Hypodermis, Seam cell, P cell, Rectum & anus

Tissue specificity (L1): Hypodermis, P cell, Rectum & anus

Number of insertion lines: 2

All strain names: SYS1484, SYS1493

Representative strain name: SYS1493

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage