microRNA name: miR-61 miR-250

WormBase ID: WBGene00003289 WBGene00003344

WormBase description of miR-61: Involved in gene silencing by miRNA and regulation of vulval development. Expressed in several structures, including P5.p hermaphrodite; P5.pa; P5.pp; P7.p hermaphrodite; and P7.pa.

WormBase description of miR-250: Is affected by several genes including daf-12; eat-2; and prg-1 based on tiling array and RNA-seq studies.

Conserved microRNA sequences of miR-250:

D.melanogaster: dme-miR-1007, dme-miR-11, dme-miR-13a, dme-miR-13b, dme-miR-2a, dme-miR-2b, dme-miR-2c, dme-miR-308, dme-miR-6

H.sapiens: hsa-miR-128, hsa-miR-27a, hsa-miR-27b, hsa-miR-499-3p, hsa-miR-768-3p

Conserved microRNA sequences of miR-61:

D.melanogaster: dme-miR-279, dme-miR-286, dme-miR-996

H.sapiens: hsa-miR-134, hsa-miR-708*

Location: Intergenic

Promoter length (bp): 1056

Promoter sequence: tagagacaaaattgatgttcggttttcggtattgtctttaataagaaaatcaatatgaactcttcgaaacttgaaatgagtgaaatattttactgataaagagtgcattaatcggcaacggaaacgaagcaattacgataaataaaagttcaagagagcaaacttaatttttttttcaataaatcaataattaaattcgttatagtttcaatacaagttcctgaaaacatattaaacggtaaattcaagaagtaagtgattatgtttgctttgaaattaacaaaaaaaaagcgaaacgtgcacttgggaatgcagaatccagcacacaattacgtcagattacagacttcacgaactaccgtagtgagcttctgcttttcaggaatttctaccgagtcaattgaaaaactctgcatgaagatttgagcaaaaaaggtcataatctatcaaggaaagtgaaaaccacattcaaaaaagaaaattgtaaattttaatgccatttttacgctttaacgcctagaatttagagatttatataatacaaattcgattttcaacaatacacgttggactattgtattttttcgaaaataaattattatctggttcagaaaacgaacaaaccaatcggcaaaacagaaaaatgagtatttggcatcgatattccaatttctaggcataaacttcctcactcgtcgattctcacatcaaataagcttgatttcgttcgatttaatgctgaaaataactttttatattatattattgtcgaaagttagagataattcacgaaattcaatgaattttcttaatttggaaaaaataaattcaaacttcaacttttcgcgcgcaccaaagtgggcggagtttcccacgacaggcgaaatttttctctgcgtctcttcacatcactgcgatgaggcgccgtctctcgtcagtattcccaccagtgatcgtgcctataaaagctgcgcaatcgcgccacttcgccattctcctcatttccgggtcacttggtggccgttctgtccccttattctgctactccctgcctaT

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Tissue specificity (embryo):

Tissue specificity (L1): Hypodermis, Seam cell, P cell, Rectum & anus

Number of insertion lines: 4

All strain names: SYS1520, SYS1521, SYS1526, SYS1531

Representative strain name: SYS1521

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view