microRNA name: miR-72

WormBase ID: WBGene00003300

WormBase description of miR-72: Is affected by several genes including daf-12eat-2; and glp-4 based on tiling array and RNA-seq studies. Is affected by multi-walled carbon nanotube based on RNA-seq studies.

Conserved microRNA sequences of miR-72:

D.melanogaster: dme-miR-31a, dme-miR-31b

H.sapiens: hsa-miR-31

Location: Intergenic

Promoter length (bp): 1840

Promoter sequence: cactcaattctcacctccatttccaatatttattgattttttctatgcttcccgcaccccaaaaattccgcgtttttctgtattttttgtgatttttgactggtatttttgatgttttcataattaatacaatggtttttattgattttttcggaaattcgaaaaagcaggcgtataaaatttgaatttaaaactttggcgccggtggccgtggccggcggccgaggatttgcggcgcgaaaatttccggccaccgcgaatttttgtgattttgggcccaaattttcgtcattctcactcattttcccatccccgaaccccgtttttttgccgaaattagttaatttctatatttttctaactatttcagtcatttttagtcgtttttagcgtagaaatcccccgaaaattgttggttttagttgagaacccatttattctcaaatttctaggctaaaattgatgatttttcgagtttttcaagagttttcagtgactcagagcaattttctagctgattctcgtagttttccgttagaattcgtgaaaaatcgcattcaaattcgaggtttttgattaaaaatgggttttttcgcttttcaactcattttctcattcctctaggctaatttcgatagtttcgatggaaaaatcgataattttcatcggtttttaggcttttctcatcaaaattcacctcaaacggcgaaataggtataaaaatggccataaaattcggttttccgtcagtttttcatgtttttcgctcaattttttgagttttttctaggattttgctggtttttagtcaatatttagcatattttctgtataatcggcgaatttcgaggttttagatggtttttagcgagtttctccgttttctatgcatttttcggatttttcttcctcaaatctaagattttttagcctactaaagtgaagttttaagcttatttagtcaagttttgcagatttagagtgatttttccggtaattttaggatttaaaggattaaatacatttccagcgttttttcctaagttttttatcgattttcggctagttttcctggttttaagcgatttttcgcaattttcgaccaatttctgaaacagtttgcaaataaaagtcaattaagccgaatttttccgaattccagctaggtttctgcaatttttagtccaaattgtcggctctctgcggaattatatcaattttctaccaattctatagtttagtccaaatttgtcaattttaggccaatttcgacgatatttttggcatctaaggtcaattaagtctaatttttccgatttccagtgagtgttctaggtttctcagcttcaattttcaaaattatatcgattttccgacctcttaactctccggatattttcagcaatgtagctgattttcggcggtcttagaacagctttttcatcagattttcgcaattttaggtcaatttagtctattttattagattttgggctaatcgcagactttcttccgttttagtttcgaaattttggaaattttttatcgatttttgcgacctcttaactcttcgccgctaaagtgggtgtgtcacggagcacagttgggcggagtcagtaggtgagcacacatagtgagggaaagtgtgcacacacacacgtagaggcacagtccttctcctctttcgtccccacgccagtatagactctcgactcatttcactttccaacgtgttgctctgctagttgtgctcttttttcgatgctcttccctctttttgtgagcaccgggctcttgtagtcatgacgattcccctattctactactactattctgctga


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Restriction site: AflII/SacII

Tissue specificity (embryo):

Tissue specificity (L1): Neuron

Number of insertion lines: 3

All strain names: SYS1437, SYS1438, SYS1474

Representative strain name: SYS1474

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage