microRNA name: miR-75

WormBase ID: WBGene00003303

WormBase description of miR-75: Is affected by several genes including daf-12eat-2; and prg-1 based on tiling array and RNA-seq studies. Is affected by methylmercury hydroxide and silicon dioxide nanoparticle based on RNA-seq and microarray studies.

Conserved microRNA sequences of miR-75:

D.melanogaster: dme-miR-281-1*, dme-miR-281-2*, dme-miR-4, dme-miR-79

H.sapiens: hsa-miR-320, hsa-miR-548a-3p, hsa-miR-9*

Location: Intergenic

Promoter length (bp): 2003

Promoter sequence: gccaaataaaagttacattttgtgccccaaagaccattaatgtacttaaatcaacggataaacgcccaatcaatgaccacaatatttattaaaaagtgataaacaaataaacaattaggttccagacgttgcaacaccgagaagttggaaaaattttgattttagctgaaaatgggccttattttgccgaactttgaattgccataactttttttttggtaggtttggaccgatttgggtctaaaaaagcaaagtctcaaatttcagtactccaccttcaattgttcctttaatatttggaatcaatccaaatgttcaccgctttaaacgaaaacggtcagcacacccgtatcagtgtcttactttcatacaaaacgtggtaccagcctattgatgcaataattcttatcagtgaaaatttacttgataactaaaattattagttttgattatacttggatttcaacgaaaaacggaacagagtacttggttttctggataataactagaaatgtggaatggttttcacaattaactgactattaaagcagcaaaaatagttcattgtagctttgattttcaagtgagctaaactattgcaacaaacttaaatatacatacgttttgaatcacatcaaaatacagataagagcatatataatttgggggaaagcctttcaaaagtcctattgtaatccggataatcaaaatgagctgatgtcgtgtatgggatttaaaaaaattagattgggactaaaatatgcagccagaattttaaattcaaattgtttttatgctctattatgataacacgataatgatgacaattagttaaacgaaacaaaacgcgctctgtacgaattgcactttgggcattaaattttcatgtcaaaaactgatcaaaatgaataacccgaagatgaaggaaataaagttgtatttatgttctagctaaactcacaatttgtggcagggtttcaaaacttgcacaagtaaaggtgcttttggtttttgttttcacgttttgaattacgtagtgtattgagttctcaatggatctttttatgagtctaattattttcaaaattgcaatgttgtgatattttgaaaacaacatttttgaagtctgtttttctggcatgaaaacaatgataacacgcagatacctcacatttgaaacagctttaatcattgactatctggatatattaaagaagataattttaatgaaacgtactatttacttgcatgtttaagctcagaaaaacactactccttatgtaagactaatatagacaagggtgaaattttcaaaatggttagcatttttgaggcagatacacaagtaccagaaatgacaataaaacaagtataaatatatcactaaaatgtttttaagcggaataaagacaacatggctggaaacatcgcctgcataccctggaagccctaactaaacttaatttaatcctaccgcctattacatttccttctcaaaaatttcttataatctgatttaacatatcctttccacagtttttgactcatatattcaaaatatgtataataataataataatagtaaagtttatgcatgcgtattgtttattccagtagtgatatatgattccaaacaccaatatcaaattcatacactagaaacgaataccagccatcaacctcattgatttggttgttcaatccagaggtctcacacaccgattttacgctaccttctgtggtcacgcccttttttagtataaaaagctgccaacgcaaaaaagaaaatcatttactaaacgccgaccttctccccacaatgtgcgccggtgtctctctgcacactccacattctctgctggtaaattgactgcggcggtggcgaccatgtatcttatcatatcgaatcagttttgctcctcagttgggcaacatactgcctctcaatgtacaccaccattcgattgataatattattcaatgcggtttttttttctactTTC


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Intestine

Tissue specificity (L1): Intestine

Number of insertion lines: 2

All strain names: SYS1423, SYS1424

Representative strain name: SYS1424

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage