microRNA name: miR-794

WormBase ID: WBGene00045328

WormBase description of miR-794: Is affected by several genes including prg-1alg-1; and alg-2 based on RNA-seq studies. Is affected by nicotine based on quantitative PCR and RNA-seq studies.

Conserved microRNA sequences of miR-794:

D.melanogaster: dme-let-7, dme-miR-963, dme-miR-977, dme-miR-984

H.sapiens: hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-196a, hsa-miR-98

Location: Intergenic

Promoter length (bp): 999

Promoter sequence: gaggggtttttcagtttttcagatactttgagcgggtagttttgacccattcgtgatgtcaattttataggctgtttgagcttgaattctacttgaaaaagaatgtcacaaaatccaagatgcctgaaaaaaccaaatggaaacacgtaaggcacaccgatggcaataaatttatctggatattcattttcaaatcgcgaaagtttaaaacttttttggggagtctaaaagatccatacaccaaagcacaaccttaccaagatcccactttcagccgctaaaagaaaaatatttttaatctacataattgcagtgcggaggtgcattttcaaaatcttgactttaacacatatcttttttgtgctctctggactcatttgtgcctgatagagacgccgatgagcaatcagtggcgggtgataagacaaaaagtcagaagacagacgacttctgtctcttgctactcgagcagttaaaaatctgcaacggtacttttccagatcaaactggaaaatcagccttgaattgtgttctagagaagccttgcaaagagtaaattgatcgaatttgatagggaacatccgtgaagatcactaaaacttctgagtttctggaattaattacagcgcacctactaaccagcaagttctccaaaccgagaattacgtcagcctttatgaaatttgagatgaatctgtgaatcttgttccaaaccgaaccaattttttgaaaatatataagtcctttcagttttccagaagtaaacttcagaaaatgtttttttcaaaaattgatccctaaagctcatttgatttctgactcctcatctcttctcctcatttcgtaaagttatcactagtcacataattgtagtacatatactctgcgtctcttcagagcagtggagcctgggagccaaggagcgaaggagcacagctcttttctcttatctttacttgcagctactaaactattctgcaaaaaaaaaCTGAGCGTGTC


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1): Neuron

Number of insertion lines: 10

All strain names: SYS1433, SYS1451, SYS1452, SYS1454, SYS1455, SYS1470, SYS1471, SYS1472, SYS1473, SYS1492

Representative strain name: SYS1473

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage