microRNA name: miR-82

WormBase ID: WBGene00003310

WormBase description of miR-82: Expressed in amphid neurons; excretory gland cell; and tail neurons.

Conserved microRNA sequences of miR-82:

D.melanogaster: dme-bantam, dme-miR-306*

H.sapiens: hsa-miR-450b-3p

Location: intron sense

Promoter length (bp): 2565

Promoter sequence: TACAGCCCTCATTCCTCGTTCCTCCGCAATAATTTTTCATAGTGTCCTTGTGTTTTTACTAGAGCATTCAGATTTCCGCCCCATAGATTTCGAAAAATATCTCAGCGCCTTGTTCTTTTGAGAACTACTAAGGCAAACATGAATCATAAAGTTTTTTTGTTGATAGTTATATTGATGTGACCATTGATGTGAGATTTTCTACAAAAAAAACCAAATCATTTTATTATTCATATTACTGTTCTCGTACCTTTATTTGAATTGCTTGTTTCTGAGCATATAAGGACATATTCTCAACAACGTAGCCCGATTCGTTTGATCAGCTAAAGTACCCACACTTCAAATACTAATATAATAGAAACACATATGAAATCTTTGAAGGTCCCAAAAACATTTCAACATACGGTCACTTATTCTGTTTAGATTTTAGATGTGTTTTTCTCCATTTCAATCAAGCCAATTTATATGCATTTGTTGATGATATTCATAACagtaatgtgaacggagttagtttatatttcatttagcatattgatttcgtacgtaaacaaatcggtcgtcaatattggcataaacaatcaaatatgaaaagtaaaggggcacatctggaaaccgaaaaaaacaggaataacaggacgagacaatgagaaagtgttgaattttttaaggtacaaacagtaaggaaattaaaaaccatttttcagaaaaattttgaaaaattttatatatagagtcaaaaagcggttttcaaaattaattttcatcacttaaaaaattatttttcttccagttttatgtaatattttttaaggctttcaatttaagatatcaattttattttctgttgcaacaatttcacagaaaaaaactatttttgtaacaaaaaacttttggcttctgcacagtcgaagactgctttacaaatgtttttcaatgactaacagcacatgcgaaacttgtacagtttttgctttcaaaattctcaaaacaactgcatgtacatgtactagcatagaaaaaaattatgttcttggaaaaaattgataaaattttctaaaaatatgacagtttttttttaatatttggtgcttgtacaccccacatttgtaaaagaaattaaatcaaaagtcttagaattccaaaataaaattgaaattcttataaaaatgtttttacctaaatattcaacataaggcttaaaatcttttttttatcagtaaaaatttcttaccgtaaaattcctaaaacaaagtgaaacttattatttttctcagtcatttttttttgaatcagtgaattatcaaattgacggtagagtggcgccagtgtggaaatggttagaacccactccgggtgttccaaattggccaaatattataatataacttttcaaaaaaatttttgaaaattttttatttactgtcaaaagatgacaattactcacaatcagtttttgccactcataattttagaaattgaccaagaaaaaaattttgaacacgccacattgctttgaatttcctcaaaagctcatgtttttcaaaaacttcgcaatttgccaatactttgaccctaggttatgaaaattctgaaactttttggagtgttgtattatgatatttagtcgttttgaatcaaaattcccacttgcgcttctccaccttcaacttttttattacagtacaaatctgttatctgtgtttttttaggtataatgaatgtgattaaattaaattttttaagttaaaaatgtagtcaaagtttattgtttttcaacaaacgcccatgacataaaatttcgatttaaattgtttccagcgttttctgttaatttatcagagctaatcaaccatatcactgtactaaaataaatacccgatcacatcattgagatttcaaaataataaattaaagatgaagtttcgtcagctcctaatgggttaaaaaatcgagtaccaatgtaaatctttcaaaaaaaaatttttttaggtcaaaatttttgaagtttttcattttggtattatgtcattttagcgaaaattgctccgttttacaatacgaaaaaatattcaataatgctcatttgtctctaatactttttgtcactgtttgttgtaatccactccgttccgatttcctacctattcagtgagggggttttgcaagggaagcttgtgtcattcaaaacttgttctattgaactttggttctttttttgcttgcaagcaatcctggagaaactgagagtttatggtatagcgagagaagcaggatgtgcgagagaaagagagagagagagagaaagcggtgttggaatcagtgcgtttgtttggaggatgaaaagaggaggcggactgtatggccccgcccatcccctcccctctcgcctccgtctttctcccggcaagtagtgctcgattcattccgtatctcaccttccatggtgaacagtcgtcctccctatcattctctctactactagtgaactcatGAAATAGGTTCTTTTAGCAACCGGTTTTCTCTGTGATCTACAGAATGACAGCTAATCGTCTGAGATCATCGTGAAAG

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Tissue specificity (embryo):

Tissue specificity (L1): Pharynx, Coelomocyte

Number of insertion lines: 3

All strain names: SYS1670, SYS1671, SYS1672

Representative strain name: SYS1671

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view