microRNA name: miR-83

WormBase ID: WBGene00003311

WormBase description of miR-83: Expressed in body wall musculature; neurons; and pharynx. Used to study Parkinson's disease.

Conserved microRNA sequences of miR-83:

D.melanogaster: dme-miR-285, dme-miR-995, dme-miR-998

H.sapiens: hsa-miR-21*, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, hsa-miR-593*

Location: Intergenic

Promoter length (bp): 1978

Promoter sequence: aatacgaatttcccaccattttgccggctttcaagtttgatttaccaaaaaagagtcaacttacggaaatttccgcaaagcctgtatacaaaatagtgcaagattattggaagaaatatggtatttgaaattaaaaattgttttaggacgaattgtaaaagggaggaagcgatttgaccgcctcgcttcccggacccccattgaattagaatcccattaacagtattactttttacgatgcccattaaaggtcgatattttgagccagtttcctaggtgaatccacattctcatttcatttcccattcacttcatccattcaaatctcttttctttctctctctctcacctgactcttttttcctttttcatccatccgccacctattctctcccgtttcccaattccattttgctctcttttcatatgtgttaattagtgctttccgtcaaatgcgcgtgcggtgtctgccgaagagagtgcacacgttgtcatggcacgtttccctgacaacgagaagcttcgctgtattcacctgccgttttctccatctgctcacaagctcactttactattcgttttggtgtgtgtgggtggtcttctactcttgattattattactcatcttcgtattgttttctgaaaaaaaaacagataaatacataaacttgtaagttttgactattttctgatttatggtcagacatgcctctccgcgacgaggcatttgttaccaaaaacatttattgatgattgattctaaatcagttgatgtaagaaaaatttcaaaaaatcaaatacggcttctccaaattcaactaactagtctagaaattgcacacgggatgccattctcttcatattttcatcatttagttaccgatggttttacggcagaattataaattgattattaaaattaactgtttcgtaaattatttaaataattatttcaaaatatttgtttatttttctttgtggaaattttaaaggcgataagcgggcgagctgacgttaacctcgttaagatgtatatgaaaacaagtgcattttttaaatgaattttgcattcaaatgttttctataacacttacagctccacttgggcagattttatctcggttatctttggttttatcaaaaaaatgtaaaacactaaaaatgcgtctcgaatatttctctatttgttcacagctgatcaatttttgacaaaaccaataataaccaagatataggctttcaaagtaaaaagcggggttccaagactaataaaaaaatacgatatgtacttatgtagaaatcaaaattttgaatttcaagtttaaatttagtgtttttcctcttgaagccaacacccaaaagaaaaacatttccctcctttctcattttttcttcgcctctttcttcttttcccgcttactcattaatttagcgtcgtgcacatcttctaaggtcctttccatttctttcttttctatctttttcttttaatttttgcagccactttcatatatacataaaaggccgaaaattcatgtttttaaatgagtgaaaagaagttgagaattcctgttgcaattaaaactgaaatttcgatctagtgcaaaattaatctacacaaaaattcattcaaaacagtttgttttgcactcatattacagtgtcactcttaaagtgcaaacatcaatcaatatattttgttatcagttacgtggaaatggcaaacagcacatacctctttctttttaccttttttcaacaaacagcacacatattagaatatgaattgaccgataaaagtgtctccgcctacttttcctcgcccatcggtgcaacaggcctcccgaactgtacacgtcgattaaacgattattgtgttggtagaagaggagaaaagttttcttcatttttctttttttgttttacttttctttcttactcgcaggtacaaattattcaatttaaaagcaccactcgg


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Restriction site: ApaI/SacII

Tissue specificity (embryo): Neuron, Intestine

Tissue specificity (L1): Neuron, Intestine

Number of insertion lines: 3

All strain names: SYS1426, SYS1432, SYS1439

Representative strain name: SYS1426

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage