microRNA name: miR-84

WormBase ID: WBGene00003312

WormBase description of miR-84: Predicted to enable translation repressor activity. Involved in several processes, including miRNA-mediated gene silencing by inhibition of translation; negative regulation of Ras protein signal transduction; and positive regulation of development, heterochronic. Expressed in several structures, including P5.ppp; gonad; somatic gonad precursor; touch receptor neurons; and vulval precursor cell. Used to study Parkinson's disease.

Conserved microRNA sequences of miR-84:

D.melanogaster: dme-let-7, dme-miR-963, dme-miR-977, dme-miR-984

H.sapiens: hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-196a, hsa-miR-196b, hsa-miR-98

Location: Intergenic

Promoter length (bp): 1943

Promoter sequence: ACTAAACAAAGTCAGATCACccccagatttttccgtcaaaaaatttcaaaaacatatgattttcaaattgctcaactttcccgccaaaaaatttgtcataaaagtggccacccatagtgattacctcctattcggtttatctaaccttttccgaaatcgttatttttctggtctgatgtgcactttttccttcgcctttccccaaaagttcaagcaaacttgaagggcaagactgcggaaaaactgtgcaaataaatttctcttcaatgatttcattgtctggaattcctcgtcgagaaatcattctagaatcttgagacgagcaaggggcgtgccctggtgatcatgaagaagccgaagagtgaagaaaagggcggagtcaaagggaagggctcaagatagatttttagtttttcattcgaagctggaaaccttcgagccaatgtattgcctgccaaatccctccagtgataagcttatttcaaatttctacacagaaagtgcgtctgccggagtttgatctatttagaaattcatagcaatatgtacgtgagtggctttttggtgtggtctgtctgggctttaaaatattttctgcagatgtaatttttcagaggaatgttttaaggttttttctagttactgcatctgtgagcttgtgtgctcctcggtaaatgtgctcgccgaacaaaaagggcttttcataattaaatcaagagaaggtgtagtttttccaattctagttcaagttgtagcctaaatagcaaaaatttgcaaaacttctagcctacatttcagtctaagtttcaacttaaattttaacgaaaattttagtttcaaatcgtagcctaaattttcatctaaattttaaagtttttcctacatttcaatttaagtctaaatatgtactaaaattaaatttaagacttggtagttttatcgtaaattgcagcattgacacaaaggcaagagcttgggttttggctcaaataagacaagatttaaacaaaactataaatttagttcaagctaaaattaaagcttagtttgtatctcaagttcagcttgaatttagtgtaagcttcaccttaaatttcaaagtatcattcagcttcaattttgtcttaaatttttacttgaactcgagttataatttgatcttgagctataatttaatttaaaaaaaatataaaccttagtttaaatctaaattcaagtttttttcaagattttctgctccgacagattaacatgtttttctccaaaaaatcttctctgcacccttctttttagatacagcacttgcttcaacaaaacatgcctgactcagacttctccatagatcaaaaaacctctctcctagtcgctggtcagtgacagtgcatgcactttggcgtatcggcggaaaaggtcattttgaacaaaaaactaacttgtttttttcctttcctcttctctcttacagccatcaccctatccctttattcttactttccactatttctatttcttcccttgtttctaattcttagtttttagttttcagttcccagtagttcctccattcgaccataaagccaagtttcaatacatagctgtacaaacggaacgtcctaccattcaaagcaccgagaaacgaacgaacgcaagcagcgaaaaaagaagaagaagaagaaaaaagatctgcgcgttcgttcctctcttctcgaggtgttctaatatttggcgatgcgagaaagtgcaattcagctagagaatgcgacatcactgtctggtttcggcggataggacaccccatcggcgtgacagtaaaaaaagggcgagcggaggcgggtaggccggtggggggcgggccctggctcctgattattctactgcagcacactttattcattcctctatattcctgagctactattcatCATACGTCTGCCTGTGGCATC

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Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 5

All strain names: SYS1766, SYS1767, SYS1768, SYS1773, SYS1775

Representative strain name: SYS1767

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.

Embryo

L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view