microRNA name: miR-86

WormBase ID: WBGene00003314

WormBase description of miR-86: Expressed in dorsal nerve cord; nerve ring; tail neurons; and ventral nerve cord.

Conserved microRNA sequences of miR-86:

D.melanogaster: dme-miR-987

H.sapiens: hsa-miR-545*, hsa-miR-559

Location: intron sense

Promoter length (bp): 2000

Promoter sequence: "atcaaaccgtgatgggaaagtccacgttgtgtcagtctgtcttaataaagtttgatctacaaaaattgcgggattttttt


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 6

All strain names: SYS2271, SYS2272, SYS2273, SYS2274, SYS2275, SYS2276

Representative strain name: SYS2271

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage