microRNA name: miR-90

WormBase ID: WBGene00003318

WormBase description of miR-90: Is affected by several genes including daf-12eat-2; and prg-1 based on tiling array and RNA-seq studies. Is affected by nicotine and sanguinarine based on quantitative PCR and RNA-seq studies.

Conserved microRNA sequences of miR-90:

D.melanogaster: dme-miR-190

H.sapiens: hsa-miR-190, hsa-miR-190b

Location: intron anti-sense

Promoter length (bp): 1219

Promoter sequence: cattgtgtttaacaatgccatgctatctgggtatttacttgggtaatttcatgaggttgaataaaatatctattatagaagaaaaagtgtatgaggtgaatgatgtagttgaaactcgttggcagtagcacttggaaaagtttgaacactagtttcgaagattcagcctattgaactttagtaggcaaactttttcatatactggaaataaaacaagatggctggaaatgcactatatttatgtaactgtaaaaattcaaaaagggacattctgaaatttgttattccaatcggagacatcatttaaacccaaacttaaaattattttttcgtagcttcggtgggaaggtgattacctcaatttaagataaacagtaccgtaaatgactcatctagtaagaatcgggaatttgaatccataccacaaatatttgaatggtaccggtaatctaattgtaaaatccaaaatctggtcgtcgtataatttccaaaatggattttttggaatggttctgaaatttgactgaatttaaattttaaaaagttgttttaattatttttttcaaccgaggtaggaattgaaaatcacgaaaatacaacgaaatttcaactccttccattttccattcattgccaaaaaagctcaattccaactactacttcttcccgtttttcgtccggaaatgattgatcgtttcccatttggccagccgtcgaagccaacagcactctctttctcccccatccttcttttccttcccaacggcccatctgctgctctggtggaagggcaccgcccgtcttctccctcccctacggaccgctcgttgcaagaatctctaatgtctgggacactctctctctctcacacacacacacacacaatctccatgccagggcacccacgacgccatcaccatcgccttgcggagacgacgattgagtgttggagggacacatgagatagtattgagaatcgagagactttgacgactgtgagtgtagagtgagcgttgtagtgagtgtagctgtgagtgtatgttttatgtttccctcctcttcccttagtctccgccctttttcctccctggcccccgtcgtccttcttttccaccagtggcaccattctctccgtattcgggtcccggtcaccctccatcttccctgacccttttattctcctttttaaattagctttccatttctaacaaaacGGGCG


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Restriction site: ApaI/SacII

Tissue specificity (embryo):

Tissue specificity (L1):

Number of insertion lines: 7

All strain names: SYS1430, SYS1434, SYS1436, SYS1446, SYS1450, SYS1490, SYS1494

Representative strain name: SYS1490

600-cell stage and L1 stage
The black dots on the lienage tree represent an increase in the fluorescence intensity of miRNAp::mNG::H2B during this cell cycle.



Embryo
L1

Embryonic 3D expression pattern

4-cell

8-cell

15-cell

28-cell

~50-cell

~100-cell

~200-cell

~350-cell

~500-cell

~600-cell

600-cell

bean-stage

L1 3D expression pattern

Left view

Right view

Dorsal view

Ventral view

Lineage expression pattern of all strains until 350-cell stage