END-3

WormBase ID: WBGene00001311

WormBase description: Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II proximal promoter sequence-specific DNA binding activity. Is involved in endodermal cell fate specification and mesodermal cell fate specification. Localizes to nucleus. Is expressed in E; E lineage cell; Ea; Ep; and intestine.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AATTCAATGAAGAAGGCTAC

sgRNA2: GATGATAATTCATATTCTGG

5'HR: GGATCCTTACCTAATTTTGAGAAATGCATCTTTTGGCTCAAAGGTGAGACTCACTGTGTAGGTCTCGACGCGAACAAAAAATATTTCGCGCAAAGGCGTGTGTTCCTTTAAATAGTACTGTCGCCAATTAATTTTTGCAGTTTCTACAGTATATCCCACGTGGTGTCAGAGTGTCCCATATCGGTTTGATCTTCGTAGATCTACGGGAATAGCGGTAATTTGACGCAGATTTCTCAACTACCAAAAATATTTGTTGGTTGAGAATTGAACAGAAAAAGAAGAAGAAAATACAAGCATGTCGAGCTTTACACAACTTTATCTAATAAATATTCAATTAATTTGGATCAGTTTTCTCTTTCAGCAATTACAAAAAATTTTGTTCCGTTAAAATTGTGAATGATTGGATTTGGAGAGAATCGAGTTTTTGGAAAAATAAAAAACAAGAGCAACGTGTTATGGTATTATTCATGTGATCTTTTAAAATGCAAACGTGTATTTTAATTTAATTGTTCCCCTATCAGAACCTCTCTAAATTTCACGTGTTTCAAATTCCCTCTTTTTCTCTCTACTCTTTCACTCTCGCACGTGAAAAACTTCTTTCCGAATGATAATTTGTGTTTAAAATGGAAATTTTATTGAAGTAAAATTTATGAAACAAATACTTTTAGTTTAAATCCGAGATGCACTTCTATTTGAGAGAGCCCACGGTAAATTAAAAAAGAAGTAAAAGTATACCAAACAAGTATGTTCCAAGTGTTCCGATGGCTCCGACAAAAGAGTATACACTGATTGTATAATCGGTTAGATGAATAGCTGAAAGTATACCACGTTCAAAGCACACCCCGCCTTGTGTCTCTTCTAGAAAAAGGCGGTTGCTGAAAAGTATACGGAACGCTGAGGCACGCCCTCTTCTTACCATGATTTGCTCCAAGATAAATGAATAGGCATTCTCATTCAAAGTATAAACGGTACC

3'HR: CTGCAGATGTACTCGAACTCATTCTCTTCATCATCTTCATCTTCCAACTCACCAATGTCTTTTGATTTTGGATTCCCTCAGTTTCCCGAACAAGTCCAATTCAATGAAGAAGGCTACGGAACACCATCTCCAGATGTTCTCCAGAATATGAATTATCATCATTATCCGGCACAAGATATGACGACAAATTCATACAATGGAGGCTATGACAATTCAATGCAACAGAATTTTATGCAACCAGATAATGGTGCAGGATACTACAGTGAGTTTTAAAAAACTATTTTATTTTACTTTTATATTTAAAATTAATGTTTCAGATGAAAACTATCAACAGATGCCTGATTTTCAATTTCCAGTTCAAAATTTTGATTTTACAAATCAATTTGAATTCACGACGCCTATTAATGGTATAATTAACTATGAAACTTACAGCCTAATCTTAAATTTATTTTAGACCTCCAGCAATCTCAAACTTCCATCAACCACACCAATCCCGACAACAATGAAAATTCCATGCCAGAGATACCAATTGACGGGGGCTTCAACTTTTTTCCAGCTCAAGAAGTTCAAGAATGGAAGCCAGCTAGAAAAGCTTCGAAAAATAAAATCAAGAAGATTTCCACAATGCATATCAACTCTTCGTGCTCCAATTGTGGATGTCGGGAAACGAAATTGTGGAGGAGAAATGAGCAAGGAGAAACTGAGTGCAAGTAAGTTTGAATTATTTTGAATGTTCTATTTAAATTACGATTACAGTCAGTGAGAAAATGTTTAAAAATCATTCCTATGCTGATAAAGTTACCAGATACCGCAATGAAACTTTTTATCATCTTTTTGAAATGTTTTATTCATGTCGAAAAGTAGTAAAAACTCTTTTTTTAACCACTTATCGAGAAATTCAAAGCATTGTCTGTTTTTTGATCGACGATTTTCAAAAAAAAATTGGTGGAAATTTTTTTCACTATTACTAGT

KI verification primers: END-3-NKIF: 5'-TTCACTTCGCCAACTTCAG-3' END-3-NKIR: 5'-ATGTCCTTTGACTCGTTCGT-3'

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Strain name: SYS436

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; end-3 (devKi109 [mNeonGreen::end-3]) V

Expression pattern: Lineage-specific, Intestine-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): END-1, ELT-7, MED-2, ELT-2, DVE-1, NHR-102, TBX-9, CEH-13, HMBX-1, TBX-11


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA