HMBX-1

WormBase ID: WBGene00018786

WormBase description: Is an ortholog of human HMBOX1 (homeobox containing 1). Is predicted to have telomeric DNA binding activity. Localizes to nucleus. Is expressed in body wall musculature; enteric muscle; somatic nervous system; and vulval muscle.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-CATCAACAACCTCGCCAATC-3'

Strain name: SYS178

Cross: OP655 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs655 (hmbx-1::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Muscle-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): CEH-22, CEH-60, DMD-4, END-3, EGL-5, CEH-51, SYD-9, VAB-7, MED-2, LIN-39, LSL-1, HLH-1, HND-1, PHA-2, UNC-120, TBX-7, M03D4.4, PAL-1, TBX-9, NHR-102, TLP-1, CEH-39, CEH-20


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: NHR-102, AST-1, DAF-19, ETS-5, K09A11.1, LIM-7, POP-1

Targeted by: ELT-2, ZIP-8, BLMP-1, DPL-1, ELT-3, PQM-1, RNT-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: DAF-19, ETS-5, POP-1

Targeted by: ZIP-8, BLMP-1, DPL-1, RNT-1