EGL-5

WormBase ID: WBGene00001174

WormBase description: Is an ortholog of human HOXA7 (homeobox A7) and HOXB7 (homeobox B7). Exhibits transcription factor binding activity. Is involved in several processes, including anterior/posterior pattern specification; nematode male tail tip morphogenesis; and regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including neurons; non-striated muscle; ray precursor cell; rectal epithelial cell; and somatic nervous system. Human ortholog(s) of this gene are implicated in several diseases, including brachydactyly-syndactyly syndrome; diabetes mellitus (multiple); and dysostosis (multiple).

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GCCACCTTCACGCCTCCT-3'

Strain name: RW10867

Strain genotype: unc-119 (ed3) III; itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10116 (his-72p::his-24::mCherry::let-858 3'UTR + unc-119(+)); stIs10867 (egl-5::TGF(7E3)::GFP::TY1::3xFLAG)

Expression pattern: Posterior-biased, Muscle-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): SYD-9, SEM-4, SNAI-1, VAB-7, VAB-15, HLH-1, UNC-120, LIN-39, M03D4.4, PHP-3, TLP-1, CEH-20, ATF-7, BAZ-2, PAL-1, HBL-1, HMBX-1, CES-2, UNC-62, CEBP-1, F23B12.7, Y75B8A.6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-14, LSY-2, NFYA-1, REF-2, UNC-42, ZIP-8, DPL-1, RNT-1, UNC-86

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-14, LSY-2, NFYA-1, REF-2, UNC-42, ZIP-8, DPL-1, RNT-1